Polymorphism of the gene pol of the bovine leukemia virus
2014
Gerilovych, A. | Stegniy, B. | Limanska, O. | Gorbatenko, S.
As a representative of RNA-containing viruses, the causative agent of bovine leukemia (BLV) is characterized by the signifi cant level of genetic variability which results in considerable information content of phylogenic studies of its populations. Aim. This study is aimed at exploring the sequence of 440 bp long fragment of the pol gene of BLV in three farms in the Eastern Ukraine. Methods. The polymerase chain reaction, sequencing and standard methods of isolating nucleic acids have been used. Results. The sequencing and the sequence analysis of the pol gene demonstrated the presence of three variants of sequences, corresponding to the isolates, identifi ed in Kharkiv and Sumy regions and the sample of the proviral DNA from the FLK-BLV culture. Con- clusions. The genetic homology of the sequences of the characterized isolates to the populations of viruses, circulating in the territory of Europe and the European part of the Russian Federation, has been established.
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