Major dietary carotenoids in human blood and faeces samples: extraction, identification and quantification by HPLC-DAD
2025
Olmedilla-Alonso, Begoña | Rodríguez-Rodríguez, Elena | Agencia Estatal de Investigación (España) | Ministerio de Ciencia, Innovación y Universidades (España) | Red Nacional de Carotenoides (España)
The analytical technique of choice for the separation, identification, and quantification of carotenoids is the high-performance liquid chromatography (HPLC) with UV/Vis detection. Here, we describe basic procedures for the preparation and extraction of carotenoids in human blood and faecal samples and their analysis using C18 and C30 columns. The methods detailed in this chapter describe the type and volume of extraction solvent used, the duration of the successive steps, and the identification and quantification system and some considerations for obtaining the best parameters of recovery, selectivity, and specificity.
显示更多 [+] 显示较少 [-]To the Spanish Carotenoid Network (CaRed), grant RED2022-134577-T, funded by MCIN/AEI/10.13039/501100011033.
显示更多 [+] 显示较少 [-]Peer reviewed
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