Prolactin stimulates prostate cell proliferation by increasing endoplasmic reticulum content due to SERCA 2b over-expression
2007
Crepin, Alexandre | Bidaux, Gabriel | Vanden-Abeele, Fabien | Dewailly, Etienne | Goffin, Vincent | Prevarskaya, Natalia | Slomianny, Christian | Laboratoire de Physiologie Cellulaire - U 1003 (PHYCELL) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN) ; Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
International audience
显示更多 [+] 显示较少 [-]英语. Prolactin (PRL) has been shown to be involved in the differentiation and proliferation of numerous tissues, including the prostate gland. Moreover, variations in [Ca2+](ER) (calcium concentration within the endoplasmic reticulum) may play a role in cell growth. However, few studies have focused on the regulation of calcium homoeostasis by prolactin. The present study evaluates the regulation of calcium pools as well as the possible role of [Ca2+](ER) variations as a signal for growth modulation by PRL. We show that PRL stimulates the proliferation of normal SV40 immortalized epithelial prostate (PNT1A) cells with a maximum effect at a dose of 100 ng/ml. We also show that 100 ng/ml PRL increases the [Ca2+](ER) when measured either by indirect quantification with Fura-2AM after application of 1 mu M thapsigargin or by direct quantification with Mag-Fura-2AM within the endoplasmic reticulum. Western blot analysis shows that the SERCA 2b (sarcoendoplasmic calcium ATPase 2b) is over-expressed in PNT1A cells treated with 100 ng/ml PRL for 24 h. A small interfering RNA SERCA 2a/b, used to down-regulate endogenous SERCA 2b expression, reduced both PNT1A cell proliferation and [Ca2+](ER). We thus identify [Ca2+](ER) and SERCA 2b as protagonists in PRL-induced proliferation.
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