A diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit
2013
Garchery, Cécile | Gest, Noé | Do, Phuc Thi | Alhagdow, Moftah | Baldet, Pierre | Menard, Guillaume | Rothan, Christophe | Massot, Capucine | Gautier, Hélène | Aarrouf, Jawad | Fernie, Alisdair R., A. R. | Stevens, Rebecca | Génétique et Amélioration des Fruits et Légumes (GAFL) ; Institut National de la Recherche Agronomique (INRA) | Faculty of Biology ; Vietnam National University | Biologie du fruit et pathologie (BFP) ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB) | Institut des Nanosciences de Paris (INSP) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire d'Informatique, Signaux, et Systèmes de Sophia-Antipolis (I3S) / Equipe BIOINFO ; Modèles Discrets pour les Systèmes Complexes (Laboratoire I3S - MDSC) ; Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA) | Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH) ; Institut National de la Recherche Agronomique (INRA) | EA 4279 Laboratoire Physiologie des Fruits et Légumes ; Avignon Université (AU)
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显示更多 [+] 显示较少 [-]英语. The regulation of carbon allocation between photosyn-thetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool. RNA interference was used to decrease ascorbate oxidase activity in tomato (Solanum lycopersicum L.). Fruit yield was increased in these lines under three conditions where assimilate became limiting for wild-type plants: when fruit trusses were left unpruned, when leaves were removed or when water supply was limited. Several alterations in the transgenic lines could contribute to the improved yield and favour transport of assimilate from leaves to fruits in the ascorbate oxidase lines. Ascorbate oxidase plants showed increases in sto-matal conductance and leaf and fruit sugar content, as well as an altered apoplastic hexose : sucrose ratio. Modifications in gene expression, enzyme activity and the fruit metabolome were coherent with the notion of the ascorbate oxidase RNAi lines showing altered sink strength. Ascor-bate oxidase may therefore be a target for strategies aimed at improving water productivity in crop species.
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