In vitro shoot regeneration potential of water hyacinth (Eichhornia crassipes [Mart.] Solms): A study on an invasive aquatic weed
2025
Thoa, Tran Thi Anh | Kiet, Do Thuong | Viet, Bui Trang
Water hyacinth (Eichhornia crassipes [Mart.] Solms) is a fast-growing aquatic plant recognized as a highly invasive weed, posing substantial economic, ecological and social threats. This study evaluates the invasive spread rate of water hyacinth through the plant tissue culture method - culturing the apical and lateral buds separately at different stages to investigate the regenerative ability of Eichhornia crassipes. [Mart.] Solms shoots. Additionally, this study evaluated the potential for sexual reproduction from water hyacinth seeds at different stages. After four weeks of culture, a double-layered MS medium (solid agar base with a liquid overlay) under aerobic conditions, supplemented with 0.75 mg/L benzyladenine (BA) and 0.25 mg/L naphthalene acetic acid (NAA), was found to be optimal for in vitro growth of water hyacinth buds and seeds. The invasive spread rate of water hyacinth is based on both vegetative propagation from buds and sexual reproduction from seeds. The strongest regeneration from the apical buds and axillary buds with stolon and rootlets reached the highest rate of 60 %. The highest germination reached 40 % from water hyacinth fruit at stage 4 - the stage when the flower turns dark brown.
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