PATHOGENIC VARIABILITY OF ASCOCHYTA LENTIS IN BULGARIA
2017
Yordanka Stanoeva | Magdalena Koleva
Ascochyta blight, caused by Ascochyta lentis Vassiljevsky, is an important foliar disease of lentil (Lens culinaris L.) with worldwide occurrence and is a serious threat to lentil production. The investigation involved 13 isolates of Ascochyta lentis. The pathogen was isolated from naturally infected lentil plants from various locations in Bulgaria, using an agar plate method. Inoculum was also producedby growing isolates on LDA. Ten plants (3 weeks old) of each of the cultivars were sprayed with a freshly prepared spore suspension (105 conidia mL-1). Disease symptoms were scored 14 days after inoculation. The virulence of the isolates was determined with the help of 11 lines and cultivars of lentil (ILL 358, ILL 5480, ILL 2429, ILL 5725, ILL 7537, Laird, Ilina, Bella, Nadejda, Naslada, Zornitsa). Based on the reaction of the used genotypes, the investigated isolates of Asc. lentis were grouped into seven pathotypes. The observations in the present study revealed a significant variation amongthe isolates of Ascochyta lentis for morphological traits as well as for pathogenicity.
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