Photofermentative Hydrogen Production from Real Dark Fermentation Effluents: A Sequential Valorization of Orange Peel Waste
2025
Brenda Nelly López-Hernández | Carlos Escamilla-Alvarado | Alonso Albalate-Ramírez | Pasiano Rivas-García | Héctor Javier Amézquita-García | Santiago Rodríguez-Valderrama | María Guadalupe Paredes
This study explores the sequential valorization of orange peel waste (OPW) through photo-fermentation using real dark fermentation effluents (DFE) as substrates for hydrogen production using Rhodobacter capsulatus B10. Three DFE types&mdash:differing in prior biocompound extraction method&mdash:and their concentrations at three levels (25, 35, and 45%) were evaluated. The highest hydrogen yield (126.5 mL H2 g&minus:1 VFA) was achieved with DFE derived from essential oil-extracted OPW at a concentration of 25%. The highest DFE concentration reduced the hydrogen yield due to intensified medium opacity and potential substrate inhibition. Kinetic modeling revealed that the Modified Gompertz and Ti-Gompertz models best described hydrogen production dynamics. This study presents the first evidence of hydrogen production via photo-fermentation using real effluents derived from OPW processing, demonstrating a novel route for citrus waste reuse within a biorefinery framework. These findings underscore the innovation and relevance of integrating waste valorization with clean energy production, while also identifying key operational challenges to be addressed.
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