Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig
2025
Eckstein, Yasmin | Kessler, Barbara | Hinrichs, Arne | Novak, Istvan | Thaden, Anne von | Lorenzen, Timo | Rathkolb, Birgit | Scholz, Armin | Blutke, Andreas | Koopmans, Sietse-Jan | Angelis, Martin Hrabĕ de | Christoffersen, Berit Østergaard | Wolf, Eckhard | Renner, Simone
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2
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