Screening and Production of Industrially Relevant Enzymes by <i>Bacillus paranthracis</i> Strain MHDS3, a Potential Probiotic
2023
Musundwa Locardia Tshisikhawe | Mamonokane Olga Diale | Adrian Mark Abrahams | Mahloro Hope Serepa-Dlamini
The digestive process and intestinal protein absorption are influenced by a variety of factors. Due to their numerous health advantages, including potential favorable effects on protein digestion and absorption, probiotics have gained increased attention in recent years. Probiotics can control the intestinal microflora, which in turn affects the intestinal bacteria responsible for proteolysis. Additionally, certain probiotics can release exoenzymes that aid in the digestion of proteins and others can stimulate the host’s digestive protease and peptidase activity. By boosting transport and enhancing the epithelium’s capacity for absorption, probiotics can also improve the absorption of tiny peptides and amino acids as well as lessen detrimental protein fermentation, which lowers the toxicity of metabolites. The present study explored the production of enzymes by <i>Bacillus paranthracis</i> strain MHDS3, a probiotic candidate isolated from <i>Pellaea calomelanos. Bacillus paranthracis</i> displayed enzyme activities of amylase (31,788.59 IU), cellulase (4487.486 IU), and pectinase (13.98986 IU) through submerged fermentation. The CAZyme analysis of <i>B. paranthracis</i> revealed 16 CAZyme gene clusters associated with cellulose, amylase, and pectinase activity. Thus, <i>B. paranthracis</i> is a promising probiotic strain that can produce enzymes with biotechnological applications.
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