A Two-Stage Cascade for Increased High-Value Product Accumulation in <i>Chlamydomonas asymmetrica</i>
2024
Joachim Hahm | Sun-Hwa Jung | Saikrishnan Kandaswamy | Prakhar Talwar | Nikita Verma | Vivekanand Vivekanand | Christoph Lindenberger
Cascade systems are used in the large-scale production of astaxanthin, facilitating a successful value-added process despite high accumulating costs. However, their application to other high-value products (HVPs), like lutein, β-carotene, chlorophylls, and fatty acids, remains unexplored. This study investigates <i>Chlamydomonas asymmetica</i> in chemostatic cultures, focusing on the impact of light and dilution rate. A two-stage cascade system is designed, combining high-light growth with low-light pigment accumulation. The results show potential for productivity improvement. Notably, the spacetime yield (STY) of Chlorophyll a increased by 20.96%, reaching 2.73 g·L<sup>−1</sup>·d<sup>−1</sup> at the lowest dilution rate. Lutein maintains a consistent concentration of 22.34 mg·g<sup>−1</sup>, while β-carotene achieves a maximum STY of 3.60 mg·L·d<sup>−1</sup>. A cascade modification with a hollow fiber membrane significantly enhances HVP concentrations—Chlorophyll b, Lutein, Chlorophyll a, β-carotene, EPS, and GLA increase 27.23%, 38.95%, 31.88%, 86.19%, 128.7%, and 57.71%, respectively. STY improvements for these HVPs range from 1.78% to 82.96%. This study offers insights into <i>C. asymmetica</i>’s response and proposes a cascade modification for enhanced HVP production and downstream processing efficiency.
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