Dynamics of zoosporic parasites in summer phytoplankton communities of the Baltic Sea

18 pages, 7 figures, 1 table, supplementary data https://doi.org/10.1093/femsec/fiaf081

Zoosporic parasites significantly influence aquatic ecosystems by infecting various phytoplankton taxa, but their interactions in brackish ecosystems remain largely unexplored. This study explores microbial communities and parasitic interactions with summer phytoplankton communities at six brackish coastal sites in the northern Baltic Sea. We hypothesized that small-scale spatial heterogeneity in environmental conditions would lead to distinct assemblages of microbial communities and phytoplankton–parasite interactions. By combining DNA metabarcoding, single-cell sequencing, and microscopy, we provide the first community-level qualitative and quantitative assessment of zoosporic parasites infecting summer phytoplankton in the Baltic Sea. Microbial communities varied significantly across sites, with salinity as primary driver of eukaryotic diversity. Chytrid fungi were the dominant parasites, infecting green algae, diatoms, and filamentous cyanobacteria, with infection rates up to 5.8% of phytoplankton biomass. Sequences from brackish chytrids clustered with those from freshwater environments, reflecting polyphyletic patterns linked to host taxa. Phytoplankton–parasite interactions were influenced by host abundance and site-specific conditions with correlation analysis suggesting broader host ranges and potential generalist behavior in some chytrid species. Additionally, an unidentified oomycete infected up to 85% of the toxic bloom-forming cyanobacterium Nodularia spp. This study highlights the ecological relevance of zoosporic parasites in the Baltic Sea and emphasizes the need for further research into their role in phytoplankton bloom dynamics

This work was supported by funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement number 730984, Assemble Plus project. This work was further supported by the German Research Foundation (projects WY175/1-1 to S.V.d.W. and WU890/2-1 to C.W.); the Research Council of Finland (340659 and 346387 to S.V.d.W.); the Beatriu de Pinós postdoctoral program of the Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya (grant number BP2020-00174 to E.A.); the Spanish Ministry of Science, Innovation, and Universities (MICINN) (Project SMART PID2020-112978GB-I00 to E.G. and A.R.) with institutional support from the “Severo Ochoa Centre of Excellence” accreditation (CEX2019-000928-S)

Peer reviewed