Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization.
2012
CAVALCANTI, A. dos S. | BRANDÃO, F. Z. | NOGUEIRA, L. A. G. | FONSECA, J. F. da | AMANDA DOS SANTOS CAVALCANTI, UNIVERSIDADE FEDERAL FLUMINENSE | FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE | LUIZ ALTAMIRO GARCIA NOGUEIRA, UNIVERSIDADE FEDERAL FLUMINENSE | JEFERSON FERREIRA DA FONSECA, CNPC.
Abstract: The objective of this study was to verify the effects of GnRH on ovulation and pregnancy of ewes subjected to a short-term synchronization of estrus. Santa Inês and crossbred Santa Inês/Dorper ewes received 60 mg MAP sponges during 6 days plus 300 IU eCG and 30 µg d-cloprostenol 24 h prior to sponge withdrawal (SW). Ewes were assigned to receive 0.9% NaCl solution (Tcontrol; n = 32) or 25 µg GnRH (licerelin, TGnRH; n = 34) 24 hours after SW. Each group was assigned to intrauterine insemination by laparoscopy (n = 25) or to natural mating (n = 41). Artificial insemination was performed with a single dose of fresh semen. For controlled mating, females were exposed to males 12, 24, 36 and 48 hours after SW. Ten females per treatment were subjected to transrectal ultrasound examination at 12-hour intervals (SW to 60 hours after). Estrous response (100.0% vs 95.2%), interval from SW to estrus (32.9±7.4 vs 29.8±6.9 hours), estrous length (37.4±9.0 vs 31.5±10.4 hours), pregnancy rates (57.0% vs 41.0%), ovulation rate (100.0% vs 90.0%), number of ovulations/ewe (1.1±0.3 vs 1.2±0.4), maximum follicular diameter (6.4±0.7 vs 6.1±0.6 mm), interval from SW to ovulation (59.1±3.5 vs 58.4±3.5 hours) did not differ between Tcontrol and TGnRH, respectively. Administration of GnRH 24 hours after SW does not improve ovulation or pregnancy rate in estrous synchronization in ewes.
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