Responses to and detoxification of esculin in white-rot fungi | Réactions à l'esculine et détoxification de cette substance chez les champignons de la pourriture blanche
2024
Bchini, Raphael | Darnet, Sylvain | de Butler, Arthur | Doan, Annick | Oliveira-Correia, Lydie | Navarro, David | Record, Eric | Morel-Rouhier, Mélanie | Interactions Arbres-Microorganismes (IAM) ; Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Biodiversité et Biotechnologie Fongiques (BBF) ; Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Aix Marseille Université (AMU) | Centre International de Ressources Microbiennes - Champignons Filamenteux (CIRM-CF) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ANR-11-LABX-0002,ARBRE,Recherches Avancées sur l'Arbre et les Ecosytèmes Forestiers(2011)
International audience
显示更多 [+] 显示较少 [-]英语. Bio-based molecules have become one of the main alternatives to traditional products for limiting the spread of harmful fungi. However, a better understanding of the mode of action of these molecules and the defense systems developed by fungi to resist them, is needed to assess the benefits/risks of using them as antifungal treatment. White-rot fungi are excellent models in this respect, as they have adapted to the hostile habitat that is wood. In fact, wood is a source of putative antifungal compounds known as extractives. In this study, we demonstrated that esculin and the aglycone form esculetin, which are coumarins found in plants and wood, reduce the growth of Phanerochaete chrysosporium, Pycnoporus cinnabarinus, Trametes versicolor and Fomitiporia mediterranea, with some differences depending on the specie. We have shown that various strategies are developed by the fungi to deal with esculin both at the extracellular level, through the involvement of laccases, peroxidases and glycoside hydrolases, and at the intracellular level, mainly by stimulating protein translation. Using comparative proteomic and metabolomic approaches, our results showed that despite the fact that the species analyzed are closely related (they all belong to the Agaricomycotina, and have the same trophic mode), their defense responses to esculin are different.
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