Facile Isolation of N1,N5,N10-tri-p-coumaroylspermidine from Korean Darae(Actinidiaarguta) Bee Pollen Extract
2025
Lee, S.G. | Lee, M.S. | Son, Y.G. | Park, S.W. | Kim, S.M. | Choi, H.M. | Im, P.R. | Woo, S.O.
Actinidia arguta ( A. arguta, Korean darae) is widely distributed as a major nectar and pollen source, and its bee pollen is known to be the most widely produced monofloral bee pollen in Korea. Phenolamides identified in bee pollen have been reported to exhibit beneficial biological functions in humans. Among them, N¹,N⁵,N¹⁰-tri-p-coumaroylspermidine (TPCS) has been associated with antioxidant, anti-inflammatory, and photoprotective activities. However, the purification of TPCS has traditionally been carried out through laborious and time-consuming chromatographic procedures, resulting in low yields and limited reproducibility. Herein, a simple and efficient approach was developed for the isolation and identification of TPCS from A. arguta bee pollen. The methanolic extract was fractionated by MPLC, followed by Sephadex purification. The purified compound was identified as TPCS based on UPLC, LC-Q-TOF/MS, and NMR analyses. Further- more, to explore the potential biological relevance of TPCS, molecular docking analysis was performed against polo-like kinase 1 (PLK1), which showed a stable binding interaction. This work provides new insights into the phenolamide composition of A. arguta bee pollen and establishes a practical method for the efficient isolation of TPCS from natural sources, thereby supporting future biochemical and pharmacological studies.
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