In the field of agro-industry, the most attention is paid to the development of eco-friendly technologies, which allow to improve the efficiency of natural resources in sustainable agriculture, preserving the environment, as well as to obtain high-quality, environmentally friendly, and safe products. Currently, organic viticulture and biodynamic winemaking are rapidly developing and demanding areas of winemaking. In light of these factors, it makes sense to cultivate grape varieties that are resistant to cold, fungal diseases, and pests (in particular, the Charentsi variety). Under different conditions, the microbiological state, physicochemical parameters, organic acids content, and color characteristics of Charentsi grapes were examined. We studied the physicochemical parameters of wines made by using different methods. The possibility of making ecologically clean rosé wines from this grape variety was assessed, using it as a raw material.

The crops variety growing in Armenia has not been fully explored yet, hence, their genetic potential has not been revealed. Therefore, study of gliadin (Gld) locus contained in cereals’ embryo will further clarify the process of allelofunds and genotypes, homozygocity and heterozigocity, genetic similarity, genetic formula and genomic analysis of studied cultivars and wild species. The research was carried out in 2021­2022 in the educational laboratory of Food Quality Control of the Armenian National Agrarian University. The samples of the studied plants were taken from the ex­situ seed collection of the National Gene­ bank of Agricultural Crops and their Wild Relatives of the “Scientific Center of Agro­Biotechnology” branch of the Armenian National Agrarian University. From the electrophoretic spectrum of gliadin (Gld) for the study and identification of the genetic diversity of the inventory samples of cereals diversity protein formulas were deciphered, allelotypes and genotypes, homozygosity and heterozygos­ ity levels were determined, and genomic analysis was performed. According to the genomic analysis of the studied cereals varieties and wild relatives using protein markers, gliadin is spread into four zones: α, β, γ and ω. The genes regulating the synthesis of different parts of gliadin of α, β, γ, ω zones, are polymorphic located in chromosomes 6B(S), 1A(S), 1B(S), 1D(S), and in the case of Alti aghaj, Voskehask and Gjulgian also in 6A chromosome. There are no genes regulating the synthesis of gliadin segments in chromosome 6D(α). Research results, as genetic markers, can be used in identification and selection of studied varieties and wild species.

Throughout the current research 20 rare plant species of the RA mountain meadow zone has been studied and the botanical, topographical, cartographical and graphical descriptions have been compiled based on the existing spatial database. At the first research phase, three rare plants growing in the regions of Kotayk and Gegharkunik were mapped in the ArcGIS environment: Inula acaulis Schott & Kotschy ex Boiss., Acanthus dioscoridis and Draba araratica Rupr. For mapping, first their distribution per communities, settlements, operational significance and soil type was studied and identified, then digital mapping and analysis were performed in the GIS (Geographic Information Systems) environment. Based on the results, it was recommended to take appropriate measures for their preservation. Further changes in the ecological condition of rare plants growing in the selected regions were studied, maps of the given areas were drawn up in the GIS environment, and analysis of plant location, growth density, distance from the settlement, functional significance of the land, threats of extinction as a rare plant species, and conservation measures was performed. The received digital maps and analysis of mapping results justify the need for the development of additional measures (legal, economic, environmental). In order to map the mentioned plant species in ArcGIS environment, sequence of actions has been developed based on which it is possible to update, adjust and complete the database of rare plant species in the mountain meadow zone of Armenia and enrich the global biodiversity repository through the GBIF system.

The implemented research was aimed to evaluate the biological and economic indicators of the studied varieties of zucchini, to introduce the most valuable and high­yielding variety into production. Expert studies were conducted in 2021­2022 in the Taghavard community of the Martuni region of the Republic of Artsakh, located at an altitude of 600m above sea level. Anna (control), Adana and Zartonk zucchini varieties were studied. Each cultivar was planted in a 50 m2 plot with four replications. In each plot, 123 plants were planted in two rows of 10 m length, with 90+70 cm inter­row and 45 cm inter­plant distance. During the vegetation period, feeding with the norm of N 45 was done twice. Breeding varieties were evaluated according to growth and development stages, duration of vegetation, resistance to diseases and pests, and yield indicators. According to research, the Adana and Zartonk have a relatively long vegetation period (72 and 73 days), and the Adana (510 cent/ha) stands out in terms of crop structural elements and yield indicators. Adana and Zartonk are relatively resistant to diseases, and the difference in pest infestation is insignificant. Adana surpassed both Anna and Zartonk in terms of fruit percentage. It exceeded the high­yield control by 135 cent/ha, at the same time, with high economic efficiency, it provided 135 cent/ha crop addition and AMD 420.000 additional profit per hectare. It is recommended to cultivate the Adana zucchini variety in the sub­mountainous zone of the Artsakh Republic.

Milk is a good source of protein, calcium, vitamins, magnesium, casein. Its advantages are the pleasant taste, dietary features and the ability to quench thirst. However, in hygienic terms, it is not without drawbacks. In particular, milk is a beneficial environment for the development of various microorganisms, including pathogens, as a result of which it can easily become a carrier of infectious diseases. Therefore, the task was set to investigate the risks associated with the transmission of a number of dangerous zoonotic infectious diseases, in particular brucellosis and tuberculosis. To this end, organoleptic and laboratory studies were carried out on 20 samples of unpasteurized milk purchased from private individuals in different administrative districts of the city of Yerevan. Through organoleptic method the color, smell, taste, texture of milk was determined. Under laboratory conditions, pH, purity, density, total acidity, bacterial contamination of milk and the presence of brucellosis antibodies and causative agents of tuberculosis were detected. Milk samples were also examined for starch and soda content (milk adulteration).

Calf coronavirus (viral diarrhea) is an endemic disease that is mainly spread by maternal infectivity, cold, wet conditions and crowded housing conditions. Feces were sampled from the rectum of 50 newborn, 3-35-day-old calves. The samples were examined by microbiological, hemagglutination and Bovine enterichek accelerated test (antibody strips) in the ANAU Research Center of Veterinary Medicine and Veterinary Sanitary Examination. Epidemiological data, manifestations of clinical symptoms, patho-anatomical changes, hemagglutination reaction and Bovine enterichek test were used to identify the infection of 3-35-day-old calves. Feces with mucus content from the rectum of calves with diarrhea (50 samples), internal organs (spleen, liver, etc.) and affected small intestines of fallen 3-7-day-old animals (3 heads) were examined.During epidemiological studies, an increase in body temperature (42 0C), anorexia, exhaustion, depression and watery-slimy diarrhea were observed in sick calves. Clots were found on the walls of the arteries of dead animals, inflammation and hemorrages were found in the lymph nods and kidneys. According to epidemiological, clinical symptoms, patho-anatomical changes, microbiological research, as well as hemagglutination reaction and Bovine enterichek rapid test data, both coronavirus and enterobacterial diseases were detected in animals at the same time. As a result, farmers and villagers suffered economic losses due to newborn calf falls. The disease was diagnosed according to hemagglutination reaction, Bovine enterichek test and microbiological methods. Morbidity and mortality rates were respectively 0.1 (10 %), 0.6 (60 %), economic loss – AMD 209 400. Based on the effectiveness of the “BolsheVac” (“БольшеВАК”) vaccine, we propose to carry out preventive vaccinations in vulnerable farms.

In the current work, a comprehensive methodology for the soil and climatic conditions of the Republic of Armenia has been developed to justify the water requirement/demand of plants in the case of drip irrigation. The proposed methodology enables to calculate the values of the individual components of the drip irrigation regime, and in practical terms, it was used to determine the water requirement of perennial plantations in the soil and climatic conditions of the Armavir and Kotayk marzes. Based on the obtained results, the crops drip irrigation norms/rates were mapped in the GIS environment. They can serve as a background for practical planning and justification of the drip irrigation water demand for various crops and contribute to the development of introduction rates of water-saving technologies in this field.

This article presents a comprehensive analysis of the state agrarian policy, laws, and legislative acts regulating the viticulture sector of RA. An analysis of the strategic documents, legislative documents regulating the sector, laws, normative legal acts, and other documents related to state policy was conducted. Obtaining results led to the conclusion that the legislative acts regulating the sector need to be updated and, in some cases, amended․ In addition, the control mechanisms of the sector have gaps in law enforcement. There is no strategic document aimed at viticulture development, and existing policy measures and programs are not properly implemented and are ineffective. The article concludes with recommendations aimed at alleviating the issues revealed by this research.

Application of fertilizers is among the prior agriculture measures to achieve sustainable potato yield in conditions of leached soils of Lori region, poor in essential plant nutrients. The field experiments established that to obtain maximum potato yield in addition to the introduction of the main mineral fertilizer (NPK) it is necessary to apply complex water-soluble fertilizer (CWF) via foliar feeding thrice during the vegetation period with 10-12 days of interval. In this case the yield amount was 286 c/ha, 40.0 % of which was large and 35.0 % were medium size. The tuber’s chemical composition was also improved. In the case of using N120P120K120 the yield amount was 261.0 c/ha, out of which 30.2 % were large and 40.5 % were medium size, while in the control variant 207.5 c/ha yield was received, out of which only 15.1 % were large and 35.9 % – medium in size.

In recent years, the successful development of dog breeding in Armenia has been hindered by various diseases, the most common of which is demodicosis. The spread of the demodicosis invasion in Yerevan is mainly due to the uncontrolled breeding of dogs, violation of sanitary and hygiene rules for dog rearing, and unauthorized sale and purchase of dogs. Lack of information about the disease among the dog owner and breeders is another important reason for the spread of the disease. Demodicosis in dogs proceeds with certain variations. The extent of invasion in autumn was 51.7 %, in spring – 34 %, in winter – (32.2 %), and in summer it was 18.5 %. The relatively low temperature of the external environment and the high relative humidity of the air are probably favorable conditions for the reproduction, growth, and development of causative agents of demodicosis in dogs. Studies have shown that the highest degree of demodicosis was observed among maternal livestock, and animals older than three months. The lowest degree of disease damage was observed in puppies up to three months old.