as sources of biologically active secondary metabolites including pesticides because of their eco-friendly nature. The present study indicated that the ethylacetate extract of the Acacia saligna leaves exhibited acaricidal activity against the phytophagous twospotted spider mite ( Tetranychus Urticae Koch ).                 The chromatographic separation methods led to isolation of a pure compound from Acacia saligna leaf ethylacetate extract which exerted acaricidal action against the tested mite with LC50 value of 74.13 mg.l-1 after 48 h. Based on chemical (acid hydrolysis) and spectroscopic (1H, C13- NMR and MS) methods, the isolated compound was identified for the first time from plants as 2-hydroxymethyl-9-hydroxy-9-methyl-undecanyl (O-α-L-arabinopyranosyl-β-D-galactopyranoside.