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HYPOCHOLESTEROLEMIC EFFECT OF SAPONIN EXTRACTS IN EXPERIMENTAL ANIMALS
2018
Amany Ali | M. Tawfik | M. Hikal | M. Tag El-Din
Hypercholesterolemia was induced by feeding of rats on a high cholesterol diet (HCD) that contains cholesterol (1%), bile salts (0.25%) and coconut butter (15%) to evaluate the protective effect of steroidal saponins extracted from fenugreek and asparagus, and triterpenoidal saponins extracted from soapwort and licorice. The rats were divided into 6 groups, and the first one was fed on a basal diet and served as a negative control group. The second group of rats received HCD without any plant extract and served as a positive control group. The other four groups of rats were fed on HCD plus the plant extracts separately at a dose of 250 mg/kg/day during the experiment period (6 weeks). The protective effect of various saponin extracts were monitored through assays of total cholesterol (TC) and triglycerides (TG) in blood, liver tissues and feces of the rats as well as other blood analyses and histological examinations of liver tissues. The data indicated a significant (P<0.05) increase in the levels of TC, TG, low density lipoprotein (LDL), very low density lipoprotein (VLDL), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyltransferase (GGT) and glucose, and a significant (P<0.05) decrease in the level of high density lipoprotein (HDL) in addition to insignificant (P<0.05) differences in the levels of total bilirubin (TB), total protein (TP), urea, creatinine and uric acid in serum of hypercholesterolemic rats (the positive control) compared to the negative control. The protective effect of various saponin extracts were established by lowering the levels of TC and TG, and obtaining the other biochemical parameters near to their normal values in serum of rats fed on these plant extracts. The data also indicate that TC and TG decreased significantly (P<0.05) in liver tissues of the rats treated with various saponin extracts compared to the positive control. Conversely, the levels of TC and TG increased significantly (P<0.05) in feces of the rats treated with various saponin extracts compared to the negative and positive controls. Histological examinations showed lower content of fats in liver tissues of the rats treated with various saponin extracts compared to the positive control. Both of steroidal and triterpenoidal saponins exhibited approximately the same efficiency in their protective effects against hypercholesterolemia. The hypocholesterolemic effect of saponin extracts may be due to the inhibition of cholesterol absorption in the intestine which led to increase of cholesterol excretion in the feces.
显示更多 [+] 显示较少 [-]ANATOMICAL AND CHEMICAL INVESTIGATIONS ON ASPARAGUS OFFICINALIS L. (ASPARAGACEAE)
2016
Harb, R. K. | El-Kobisy S. | Sally F. Desoukey
Although Asparagus (Asparagus officinalis L.) is chiefly known as a vegetable herb, little information about the botanical characteristics of such plant are available. Therefore, it is aimed in this study to bring light more information about the morphological, anatomical and some important chemical contents of vegetative and reproductive organs of the plant, throughout the consecutive stages of its entire life span under the local conditions. Seeds of Asparagus (cv. Mary Washington 500 W) were sown 2nd Feb. 2013. The field experiment was carried out at the Experimental and Research Station of Fac. of Agric., Cairo Univ., Giza, Egypt during the two successive growing seasons 2013 and 2014. Asparagus is a herbaceous, perennial plant that grows up to 1.5- 2 m height. The plant posses aerial stems (ferns) and much- branched feathery foliage. The leaves are triangular scales like, 3-7 mm in length. The cladodes (modified stems) arise in the axis of scale leaves . Cladodes are found in fascicles (3-6) on each node. The flowers are bell- shaped, greenish –white to yellowish, 4.5-6.5mm long, with 6- tepals,single or in clusters of 2-3. Anatomical studies were carried out for various organs of Asparagus plant including, apex of the aerial stem, visible internode below shoot apex, median portion of the aerial stem, the cladode , median portion of spear, scale - like leaf, rhizome, adventitious root. The major chemical metabolites contents of spear were determined.
显示更多 [+] 显示较少 [-]Comparative study on growth and yield of F1 and F2 Asparagus variety UC 157
1996
Okasha, A.K. | El-Gizawy, A. | Ragab, M.I. | Hassan, N.A. (Ain-Shams Univ., Cairo (Egypt). Faculty of Agriculture)