Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diode laser light with a total output power of 3 mW on epididymal camel spermatozoa quality at different exposure times of 0 (control, non-irradiated), 2, 4, 6, 8, and 10 min. Following irradiation, the percentages of motile spermatozoa, storagability, viability, and acrosomal damage were assessed of the epididymal camel spermatozoa stored at 5°C for 4 days. Epididymal spermatozoa was diluted with lactose-yolk-citrate (LYC) extender. The obtained results showed that the highest (P<0.05) value of the percentages of motile and storagability of spermatozoa was recorded with spermatozoa exposed to 6 min of laser irradiation and the lowest (P<0.05) value was recorded with the control group. Otherwise, the highest (P<0.05) value of the percentages of dead and acrosomal damage of spermatozoa was recorded with spermatozoa exposed to 10 min and the lowest (P<0.05) value was recorded with 2 min. The advancement of storage time at 5°C decreased (P<0.05) the percentages of motile and storagability of spermatozoa, while increased (P<0.05) the percentages of dead and acrosomal damage of spermatozoa during storage at 5°C for 4 days. Consequently, enhancing the artificial insemination program can be achieved using the laser irradiation which is considered a cost-effective technique for improving semen quality. The profitable effects of laser irradiation on epididymal camel spermatozoa quality raised the motile spermatozoa, storagability, livability, acrosomal integrity which consider an indicator to improve mitochondrial function which extends the survival of spermatozoa