This study was designed to investigate the influence of in ovo injection of peppermint (Mentha piperita) essential oil (PO) on hatchability (%), antioxidant states, and some biochemical parameters of broilers at hatch. Fertilized eggs (120) were divided into six groups, the control group and other groups injected with 10, 50, 100 and 150 μL/ml of PO in saline solution on the 18th day of incubation in an air sac. GC-MS indicated that PO contains L-menthone (32.7%), menthol (29.34%) and pulegone (9.63 %) as major components. The results revealed that injection of 10 µL/mL of PO increased the hatchability compared to other groups while the body weight of the chicks was not significantly different. Antioxidant activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) significantly increased in injected groups compared to the control group. In addition, plasma total protein and globulin levels increased while the albumin/globulin (A/G) ratio was reduced. Lipid profile indicated that plasma cholesterol and triglyceride levels were significantly decreased in all PO-treated groups. In conclusion, in ovo injection with 10 µL/mL of PO showed positive effects on hatchability (%) and the viability of post-hatch chicks, without indicated harm to the blood constituents.

This study was designed to evaluate the effects of testosterone propionate (TP) on hatchability, chick measurements and some plasma metabolites in Dokki- 4 strain of chickens during embryogenesis stage. A total of 350 fertile eggs (45-55 g. in average) were individually weighed and incubated .At the 5th day of incubation, eggs were divided into 3 experimental groups as follow; control(C) non-injected groups ,while the other groups T1 and T2 eggs were injected in albumin with 25 and 100µg TP solvated in 50µl plant oil.  Results showed that hatchability % was significantly decreased in-ovo injected groups by 1214%than control groups, while embryonic mortality rate was increased by in-ovo injection with TP. Chick weight, total protein, albumin, calcium and phosphorus levels were significantly increased in TP injection than control .Also, male chicks % based on the visual examination was increased by TP in –ovo injection than control.   In conclusion: in-ovo injection of TP at the 5th day of incubation with 25 and 100µg TP/50µl plant oil had decreased the hatchability %, but increased embryonic mortality, some plasma constituents, male% ,chick weight and chick length in Dokki-4 strain of chickens.

This study aims to use one thermochemical storage materials system as a heat source for poultry egg incubation. Three types of activated thermochemical storage materials (TCMs) were used as an energy storage medium. These materials are Silica gel self-indicating (blue/pink), commercial white Silica gel, and Natural Zeolite. An open thermochemical system was applied inside a poultry egg incubator. The TCMs were humidified by the vapor produced from the evaporation of the water by an ultra-sonic humidifier at atmospheric pressure. The thermal recovery process aims to release the stored energy in TCMs. Two types of poultry egg incubator used during this study. The first one was the traditional poultry incubator (electricity incubator). The traditional egg incubator dimensions were 57×66×59 cm with capacity of 125 hen egg- 76 hatching. The second one was the prototype of a thermochemical poultry egg incubator. The thermochemical poultry egg incubator contained sensors to control and measure the temperature and humidity inside the incubator. The designed incubator egg dimensions were 56x39x28.5 cm, with a capacity of 25 eggs. The total heat losses by the wall and ventilation were 9.8 and 1.5W, respectively. The heat production by 25 eggs due to metabolic activities was 3.65 W. The total energy needed for the incubation process was 36.09W. The container of storage material with dimensions of 35x35x5 cm with 3.5 kg capacity allocated inside the incubator. The container's surface is covered with aluminum sheet with 0.5 mm thickness supplied with fins. The egg tray is placed on the aluminum sheet, and the eggs were placed horizontally in the tray. The energy consumption for traditional and thermochemical incubators for 21 days was 19.25 kWh and 11.2 kWh, respectively. The energy saving by thermochemical prototype incubator was 41.8%. The percentage of hatchability for traditional and prototype incubator was 80.9% and 71.4%, respectively.

This study was designed to investigate the influence of in ovo injection of peppermint (Mentha piperita) essential oil (PO) on hatchability (%), antioxidant states, and some biochemical parameters of broilers at hatch. Fertilized eggs (120) were divided into six groups, the control group and other groups injected with 10, 50, 100 and 150 μL/ml of PO in saline solution on the 18th day of incubation in an air sac. GC-MS indicated that PO contains L-menthone (32.7%), menthol (29.34%) and pulegone (9.63 %) as major components. The results revealed that injection of 10 µL/mL of PO increased the hatchability compared to other groups while the body weight of the chicks was not significantly different. Antioxidant activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) significantly increased in injected groups compared to the control group. In addition, plasma total protein and globulin levels increased while the albumin/globulin (A/G) ratio was reduced. Lipid profile indicated that plasma cholesterol and triglyceride levels were significantly decreased in all PO-treated groups. In conclusion, in ovo injection with 10 µL/mL of PO showed positive effects on hatchability (%) and the viability of post-hatch chicks, without indicated harm to the blood constituents.