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Microbial production of thermoalkaliphilic enzymes from El-Khorma governorate for application in biodetergent technology
2011
Bayoumi, R.A. | Atta, H.M. | El-Sehrawey, M.H. | Selim, Sh.M.
Detergents are an undetectable source of pollution, which hidden in most of our daily activities. Detergents could cause harmful effects before they are completely degraded. It is wise to reduce the amount of detergents that usually used by invention new bio-friendly formula contains efficient enzymes such as protease. Screening studies were carried out for one hundred and fifty bacterial isolates with respect to their ability to produce protease(s), after growing on slaughter house wastes (SHW) isolated from EI-Khorma governorate,Taif, Kingdom of Saudi Arabia (KSA) at 55°C, and pH 9. The most potent thermophilic bacterial isolate concerning of alkaline thermostable protease(s) production was identified as Bacillus licheniformis EGT50. Alkaline thermostable proteases productivity by the most potent bacterial isolate was affected by substrate concentrations (solid substrate), carbon source, nitrogen source, amino acid supplements, incubation temperature, incubation period, and inoculum size. Maximum both enzymes production by B. licheniformis EGT50 was obtained on SHW concentrations, 7.5 %; galactose; diammonium hydrogen phosphate; arginine at 55°C for 72 h. when inoculated by 0.5 ml. The protease production under all optimal conditions was increased many folds from 563.68 to 17825 U/ml (31 fold). The purification fold of B. licheniformis EGT50 alkaline thermostable protease increased to 394.7 after applying Sephadex G200 column chromatography techniques. The enzyme productivity of protease has been determined and the result proved the possibility to use the crude and purified enzymes in biodetergent technology.
显示更多 [+] 显示较少 [-]axonomical and biochemical studies on some Halotolerant actinomycetes isolated from sandy soil in Egypt
2000
Mohamed, S.H. (Ministry of Agriculture, Cairo (Egypt). Soil, Water and Environment Research Inst.)
ydrolytic enzyme profiles of lactococcus lactis ssp: lactis isolated from Laban Rayeb
2000
Roushdy, I.M. (Ain-Shams Univ., Cairo (Egypt). Faculty of Agriculture)
Purification, serology and some molecular properties of an Egyptian isolate of banana bunchy top virus
1999
Abdel-Ghaffar, M.H. (Ain-Shams Univ., Cairo (Egypt). Faculty of Agriculture)
Studies on some fungal diseases infecting the brood in honey bee colonies
Elbassiouny, A.M. | El-Banby, M.A. | Gomaa, A.A. | Ali, M.A.M.Ain-Shams Univ., Cairo (Egypt). Faculty of Agriculture)
