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Comparative Studies on Gene Expression of Rice and Wheat in Response to Fungal Infection
2019
Nermin Mohamed
Cereals are the foremost necessary foods for growing population of human. Wheat (Triticum aestivum) is the first important and strategic cereal crop for the majority of world’s populations. It is the most necessary staple food. Although rice (oryzae sativa) has the second place because of planted area but it serves as the most important food source for Asian countries. Wheat and rice production may be severely restricted by biotic and abiotic constraints. Disease is the major biotic stress in several regions.Comparative sequence analysis is a powerful tool to study homologous gene families, define conserved gene functions between orthologs, and identify lineage- and species-specific genes. Most annotations of newly sequenced genomes are based on similarity with sequences for which functional information is available. Apart from conserved sequences, inter-species differences provide important clues about evolutionary history and species-specific adaptations. In our study, two RNA-sequencing data sets of resistant variety of wheat (Triticum aestivum L.,) after infection with leaf rust fungus, Puccinia triticina and resistant variety of rice (Oryza sativa L.,) after infection with blast fungus, Magnaporthe oryzae were compared. 31768 up-regulated genes in wheat and 3902 up-regulated genes in rice were filtered according to fold change more than 3 and removing variants, 250 upregulated genes of wheat and rice were aligned and phylogenetic tree was generated. The result of phylogenetic tree showed close relationship between ten aligned gene pairs of wheat and rice. Two pairs of aligned gene pairs were selected randomly, super family of these pairs were obtained, the result showed that each aligned pair of proteins shared the same protein family and the same annotation and all pairs participate in plant defense pathways. Then, the gene expression of the two pairs were validated by Real-time PCR after infecting wheat with Puccinia triticina and rice with Magnaporthe grisea. Each aligned pair of the two pairs shared the same manner of expression with few exceptions in rice.
显示更多 [+] 显示较少 [-]EFFECT OF IRRIGATION SYSTEMS IMPROVEMENT ON WATER UNIT PRODUCTIVITY UNDER NORTH DELTA REGION CONDITIONS OF EGYPT
2019
Khaled Shabaan | M. Galal | T. Elgamal
Several field trials and laboratory studies were conducted to evaluate the impact of the implementation of the activities and processes of the development of the field irrigation systems at ElMahmoudia area, El-Beheira Governorate, Egypt, during the successful growing seasons winter 2016/17 and summer 2017 to investigate the effect of irrigation systems improvement projects on water productivity. The measurements were conducted in a tertiary canal (Mesqa) at the head of Nekla canal (Arafa Mousa Mesqa). Water consumption values for different crops were calcaulated, and water application was calculated through calibrating the capacities of the pumps and recording the operation hours. The most important results were as follows: Applied irrigation water decreased after irrigation improvement. Applied irrigation water for wheat, rice, maize, sweet potatoes and watermelon before irrigation improvement were higher than the corresponding values after irrigation improvement by 9.0%, 15%, 11%, 15% and 10% respectively. The productivity of wheat, rice, maize, sweet potatoes and watermelon increased after irrigation improvement by 7.0%, 4.0%, 3.0%, 8.0% and 9.0% respectively. Water productivity increased after irrigation improvement, and the increase ratios for wheat, rice, maize, sweet potatoes were 14.0%, 16.0%, 13.0%, 20.0%, and 18.0% respectively.
显示更多 [+] 显示较少 [-]POSSIBILITY OF USING AGRICULTURAL WASTE IN EGYPT "A CASE STUDY IN GHARBIA GOVERNORATE"
2019
Neveen Aly | M. El-Eraky | Salwa Abd Elmoneim | M. El-sawy
It is estimated that the rice cultivated area in Egypt is estimated at about 1.4 million feddans producing about 3 million tons of rice straw. The same estimates indicate that the cultivated area of summer maize is estimated at 2.1 million feddans, which produces about 5.5 million tons of woodland (in 2013), Most of which are burned and not the maximum benefit. The problem of research is to increase the amount of agricultural waste, especially for rice and maize crops, and not to achieve the economic efficiency of these quantities of agricultural, And the study of the development of the area cultivated for each of the maize, cotton, sesame, sorghum, lupine, black beans, sugar beet, rice and the total of these crops in Egypt during the period (2000-2016) the annual average of about 324.44, 460.06, 72.66, 354.76, 4.07, 1471.2, 298.88, 1452.41, 3114.4 and the total area of the maize crop during the study period. The statistical significance of these equations was proved, while the sesame yield was not substantiated. The development of the production capacity of the most important plant waste was studied in Egypt during the period 2000-2016. The average annual production capacity of the maize, cotton, sesame, sorghum, lupine, peanut, sugar beet, rice straw, These crops are 5586.12, 871.06, 94.47, 4.53, 235.65, 1711.65, 3217.06, 3217.06 and 2634.6 tons respectively during the study period. The general trend equations showed a decrease in the production capacity of cotton, sorghum and thermos. While the production capacity of each of the sesame, peanut bean and sugar beet was increasing. The statistical significance of these equations was not proved, nor was the statistical significance of the production of maize, rice straw and total production capacity of these crops. The cost of feeding was about 13505.1 pounds, representing about 78.3% of the total average cost of the head, representing about 96.1% of the total average variable costs of the head of about 14049.2 pounds, and amounted to about 17574.8 pounds without the use of silage, which Indicates that the use of silage in nutrition leads to a reduction in feeding costs by about 4069.7 pounds per year, This is due to the lower daily costs of feeding the head of cows when using silage in feeding. The cost of producing a ton of milk for dairy cows that used silage was about 3849 pounds, while the cost of producing milk for the head of dairy cows that did not use silage for about 13715.1 pounds, The production of milk is about 2805.4 kg, and thus the cost of producing a ton of milk for dairy mixtures that did not use silage about 4888 pounds, due to lower feeding costs for animals that used silage than not used silage in feeding.
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