The protective effect of folic acid (FA) on he-patic oxidative stress in aged rats exposed to oxi-dative stress by supply drinking water with 1% H2O2 (v/v) was studied. Rats were divided into two groups, the first group was considered as standard one (F1) which was fed on basal diet and administrated 1 mg FA/ Kg body weight (BW) daily by stomach tube without addition H2O2 in drinking water. The second group was divided into five subgroups, the first subgroup was the control (F2) which fed on basal diet free from folic acid (FF) with 1 % H2O2 in the drinking water, while other groups from F3 to F6 were adminis-trated different concentrations of folic acid (1, 20, 40 and 80 mg FA/ Kg BW), respectively. Weight gain, total feed intake, feed efficiency, liver weight and its relative weight were estimated. Bi-ochemical assay: activity of antioxidant enzymes system such as superoxide dismutase (SOD), cata-lase (CAT); lipid peroxidation level as malondial-dehyde (MDA), hydrogen peroxidase (H2O2); and liver functions [alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phos-phatase (ALP) and gamma glutamyl transferase (γ-GT), and lactate dehydrogenase (LDH)], were determined. Additionally, total protein (TP), al-bumin, globulin, A/G ratio, total bilirubin, direct bilirubin, indirect bilirubin; and kidney functions [creatinine, urea, and uric acid]; and lipid profile as [total cholesterol, HDL-cholesterol and LDL-cholesterol, and triglycerides] were also, carried out. The obtained results showed accumulated weight gain which significantly increased in rats group administrated 40 and 80 mg/ Kg BW/d folic acid. No changes in relative liver weight among tested rat groups were recorded. No significant difference was observed in lipid profile, LDH and SOD between groups (F1 and F6). Data also indi-cated that F1 group recorded the best one which was low in MDA and high in CAT, followed by F6. Folic acid showed no effect on kidney func-tions. No histopathological changes were observed in liver of rat groups administered 40 or 80 mg folic acid / Kg BW/d, thus indicating that supple-mentation with high doses of FA had a protective effect from the hepatic oxidative stress in liver of tested rats.