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MORPHOLOGICAL CHARACTERIZATION, POLLEN GRAIN FERTILITY AND SOME CHEMICAL CHARACTERS OF SELECTED MANDARIN (Citrus Spp.) VARIETIES 全文
2008
Neveen A. Hassan | Abeer A. El-Halwagi | R Khalaf | H Sayed | A A El-Homosany
The present research aimed to characterize physical and morphological features of thirteen varieties of mandarin as one of the National Gene Bank and Genetic Resources (NGBGR) objectives in Egypt which include also, collection, conserva-tion, characterization, and evaluation of agricul-tural genetic resources. The present study investi-gates the morphological characterization, pollen grain fertility and sterility as well as fruit chemical characterization (vitamin C, PH, T.S.S and acidi-ty) of 13 mandarin varieties (Citrus sp.).Including Balady, Malawy, Chine mandarin, Clementine, Celiopatra, Centra, Satsuma, Tangerine Dancy, El-Shorbagee, Sonbol, Sayed Marri, Abd El-Razik and Aswan. The Physical and morphological char-acterization descriptors revealed differences among varieties. Characterization of tree shape resulted in eight obloid varieties and five were spheroids. Leaf lamina shape morphological char-acteristic studies showed one elliptic variety, three ovate and 9 lanceolate. Fruit shape studies indicat-ed that five varieties were pyriforum, five obloid and three were spheroid. Fruit apex shape demon-strated that seven varieties were depressed, while six were truncate. Differences in flavedo (skin) color among varieties revealed that seven were orange; two were dark orange and one variety for each of (light orange, green yellow, dark yellow and yellow). As to the color of the pulp (flesh), results indicated that two varieties were yellow, 10 were orange and one was orange-red. The average number of carples per fruit ranged 10-14 carple in 9 varieties and from 5-9 carple in four varieties. The average number of seeds per fruit revealed that five varieties had ten to nineteen seeds; three varieties hold 20-50 seeds and two varieties con-tained five to nine seeds, one variety included one to four seeds, while two varieties had no seeds. Other morphological studies are investigated and will be presented. Fruit chemical characterization disclosed that Malawy had the highest vitamin C concentration (44.82 mg/100 ml) whereas both of Celiopatra and Satsuma showed the lowest con-centration (15.0 and 14.0 mg/100 ml, respective-ly).The greatest pH was determined in Abd El-Razik (pH 3.91) variety in contrast to the more acidity (pH 3.12-3.77) found in El-Shorbagee and Chine mandarin juice, respectively. The best total soluble solids were determined in the Abd El-Razik (15.1%), whereas the least ones were found in Satsuma (9.13%). Chine mandarin recorded the highest titratable acidity of 3.88% in contrast to 0.67 % for Sonbol. Studies of pollen grain fertility showed that Celiopatra and Sayed Marri varieties gave the highest pollen fertility (99.84%and 99.44% respectively). Contrarily, the highest pol-len sterility was found in Clementine variety (8.41%).
显示更多 [+] 显示较少 [-]CHEMICAL COMPOSITION OF CAMEL'S COLOSTRUM AND MILK IN UNITED ARAB EMIRATES 全文
2008
Rea H. Omer | A.H Eltinay
The chemical composition of 350 samples from camel's colostrum and milk in United Arab Emirates were studied. Results of colostrum showed 6.19 for pH, 1.041g/ml for density, 3.1% for fat, 4.95% for protein, 4.4% for lactose, 12.88% for total solids, 10.55% for non fat total solids, 1.11% for ash, 87% for moisture .For ma-ture milk, data were 6.6 for pH, 0.133% for acidi-ty, 1.028g/ml for density, 3.2% for fat, 2.4% for protein, 4.6% for lactose, 11% for total solids, 7.88% for non-fat total solids, 0.88% for ash, and 88.9% for moisture. The minerals contents in camel colostrum were (mg/100mg) as follows: 103 for calcium, 15.4 for magnesium, 14.0 for sodium, 128 for potassium, 0.25 for iron, 0.17 for copper and 43.4 for inorganic phosphate. Elements in mature camel milk were (mg/100mg) as fol-lows: 74.67 for calcium, 3.4 for magnesium, 42.36 for sodium, 136.64 for potassium, 0.18 for iron, 0.04 for copper, 0.02 for manganese, 0.3 for zinc and 38.4 for phosphorus.
显示更多 [+] 显示较少 [-]USE OF ESSENTIAL OILS FOR CONTROLLING BACTERIAL ANGULAR LEAF SPOT DISEASE OF CUCUMBER 全文
2008
Gomah A A
Activity of commercially and purified essential oils of aloes, anise, black cumin, caraway, carna-tion, cumin, fennel, garlic, jojoba, lavender, onion, rue and thyme were tested against growth of Pseudomonas syringae pv. lachrymans, caused bacterial angular leaf spot disease of cucumber, in vitro and in vivo. Three essential oils had an inhib-itory effect, based on the determination of the min-imum inhibitory concentrations (MICs 0.25-2.0%). Carnation, fennel and thyme were inhibito-ry at MIC 0.5%. The three mentioned oils showed considerable variation in the inhibitory effect by the disk diffusion method, though the recognized similarity in fumigation treatments. The greatest activity was recorded with thyme (18mm) followed by carnation (15 mm) while fennel showed the low-est effect (9mm). Soaking cucumber seeds of differ-ent cultivars in 2% emulsified oils for a period rang-ing from 0.25 to 4 hours significantly decreased the percentage of germination. Shorter duration of seed soaking, 0.25 to 0.5 hours, showed lower deleterious effect on germination and fennel oil showed greater harmful effect. In greenhouse experiments, the three oils significantly controlled the disease incidence compared to the check treatment, being more pro-nounced for thyme oil followed by fennel and carna-tion oils. Analysis of the essential oils in concern showed eugenol, dieugenol, linolenic acid, methyl chavicol (estragol), anisole and thymol, as an active ingredients.
显示更多 [+] 显示较少 [-]EFFECT OF VAPOR GARD AND CALCIUM CHLORIDE TREATMENTS ON KEEPING QUALITY OF NAVEL ORANGES AT DIFFERENT STORAGE TEMPERATURES 全文
2008
Hanafy Ahmed A.H | H.M. Rashad | Samia, G. El-Oraby | F El-Wakil
The experiments were conducted in two suc-cessive seasons (2004 - 2005) on Washington Na-vel orange fruits to study the effect of cold storage conditions on reducing postharvest losses and im-proving keeping quality for the export mar-ket.Fruit samples were obtained from El–Fayoum governorate, Egypt and treated with vapor gard (VG) 2%, calcium chloride (CaCl2) 4% or vapor gard 2% + calcium chloride 4%. Fruits were stored at 2 or 8 ºC for 60 days and the last group was stored at 2 ºC for 30 days and transferred to be stored at 8ºC for another 30 days at R.H. 85-90%.The results revealed that during storage fruits treated with Vapor gard (2%) alone or with calci-um chloride (4%) + Vapor gard (2%) had been in good quality as well as it caused a pronounced increase in peel color and fruit firmness, while fruit weight loss and juice percentage were de-creased. In addition, it caused a significant in-crease in ascorbic acid, total sugar and calcium concentration, but there is no significant effect in T.S.S. / acid ratio. While, free amino acid, total soluble phenols and free proline concentrations were decreased. Furthermore, the fruits storaged at 2º + 8ºC caused a pronounced increase in fruit firmness, while fruit weight losses, juice percent-age and peel color were decreased. Also, it caused a pronounced decrease in T.S.S. / acid ratio, total sugar, total free amino acid, total soluble phenols and free proline concentrations, but there is no significant effect on calcium concentration. In addition, there is an increase in ascorbic acid con-centration. Generally, the results revealed that Vapor gard either alone or combired with calcium chloride dipping treatments and stored at 2º + 8ºC had better results in improving fruit quality and decreasing total fruit losses compared with control or calcium chloride treatment alone at other stor-age temperatures and this was also accompanied by changes in various metabolic and physiological processes of orange fruits.
显示更多 [+] 显示较少 [-]STUDIES OF PHENOLIC COMPOUNDS AND PROTEINS AS A MARKER OF THE BIODIVERSITY OF VICIA FABA L 全文
2008
Belattar R | Merghem R | Boudour L
A collection of 12 varieties of broad bean (Vicia faba L.) was studied. This was curried out to see the variation of phenolic compounds (tannins) and proteins of the seeds of Vicia faba L. This study gave the following results - At the biochemical level; according to our results one observed a high content in proteins within the species of Vicia faba L. - The electrophoresis of total proteins confirmed the richness of proteins of Vicia faba L [albumins (67 kDa), Globulins, Vicilline (50 kDa)]. - Finally the phytochemical analysis (phenolic compounds) allowed us to confirm the richness of the colored seeds in phenolic compounds (condensed tannins) "949 T (183±9.66), Aquadulce (132.19±1.53)". The effect of time (the factor of the environment) inflates on the coloring of the seeds (oxidation) and on the polymerization of tannins.
显示更多 [+] 显示较少 [-]OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN 全文
2008
Rawia F. Gamal | Al-Fateh R. | Abeer Mohammed | M. El-Sawy
A recombinant strain of Escherichia coli harboring a plasmid containing the Bacillus thuringiensis δ-endotoxin synthesis gene, was tested for its efficacy to synthesize δ-endotoxin, in a complex medium containing sucrose and yeast extract. Also, the recombinant E. coli strain was tested for its efficacy against the 2nd instars of Spodoptera littoralis. The recombinant strain of E. coli showed a good activity against the 2nd instars of S. littoralis, the mortality was 70 % after 7 days at room temperature. A high cell biomass (8.8gL-1) and δ-endotoxin concentration (6.8 mg L-1), were obtained by the shake flask culture (100 ml medium/250 ml flask, at 200 rpm), of the recombinant E. coli in modified MR medium containing sucrose (20g/L), as carbon source and yeast extract as nitrogen source, in the presence of CaCO3, K2HPO4, MgSO4, FeSO4 and ZnSO4 as mineral salts. The best pH values for cell biomass production and endotoxin production were 7.0 and 7.5, respectively. The corresponding figures for the best temperature were 37oC and 30oC, respectively. The use of some byproducts such as black-strap molasses, corn-steep liquor and cheese whey, as an alternative for carbon and nitrogen sources of medium, were found to enhance the cell growth but showed no effect on endotoxin production.
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