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Preliminary study for testing the significance of butyl–iso–butyl phthalate in controlling faba bean broomrape
2019
Hani Saudy | Mohamed Hamed | Ibrahim El–Metwally | Khaled Ramadan | Karam Aisa
Due to deleterious impacts of broomrapes against the host plants, its combating is essential for sustaining crop productivity. Thus, two–year field trail was conducted at El Nubaria experimental farm, National Research Centre, Egypt, on two faba bean genotypes (Misr–3 and Sakha–1) to investigate the importance of butyl–iso–butyl phthalate as an activator for broomrape germination in comparison to glyphosate and unweeded. Findings revealed that glyphosate in 2015/16 and 2016/17 seasons in addition to butyl–iso–butyl phthalate in 2016/17 season caused significant reductions in broomrape biomass and numbers of broomrape–infected faba bean plants plot−1 compared to the unweeded. Plots of Misr–3 genotype treated with butyl–iso–butyl phthalate in both season as well as plots of Misr–3 or Sakha–1 sprayed by glyphosate in the first season achieved the maximum seed yield of faba bean. Since butyl–iso–butyl phthalate exhibits suicidal germination of broomrape seeds, it can be exploited as an effective and helpful tool in integrated management programs of broomrape in faba bean fields. Due to deleterious impacts of broomrapes against the host plants, its combating is essential for sustaining crop productivity. Thus, two–year field trail was conducted on two faba bean genotypes (Misr–3 and Sakha–1) to investigate the importance of butyl–iso–butyl phthalate as an activator for broomrape germination in comparison to glyphosate and unweeded. The trail design was a strip–plot in completely randomized block design in six replications. Genotypes occupied the vertical main plots as well as broomrape control treatments distributed in horizontal ones. Results showed that broomrape biomass and numbers of broomrape–infected faba bean plants plot−1 markedly responded to weed control and genotype and their interaction in both seasons, except numbers of broomrape–infected plants of faba bean genotypes plot−1 in 2016/17 season. Also, findings revealed that glyphosate in 2015/16 and 2016/17 seasons in addition to butyl–iso–butyl phthalate in 2016/17 season caused significant reductions in broomrape biomass and numbers of broomrape–infected faba bean plants plot−1 compared to the unweeded. In plots of Misr–3 or Sakha–1, glyphosate was the potent practice for reducing broomrape biomass and numbers of broomrape–infected faba bean plants plot−1 in 2015/16 and 2016/17 seasons, with no significant differences with butyl–iso–butyl phthalate application in 2016/17 season. There were no noticeable variations between weeded practices and genotypes and their interaction on total dry weight of faba bean plants estimated at 105 DAS, in both seasons, except glyphosate x Misr–3 interaction in the first season only. Plots of Misr–3 genotype treated by butyl–iso–butyl phthalate in both season as well as plots of Misr–3 or Sakha–1 sprayed by glyphosate in the first season achieved the maximum seed yield of faba bean. Since butyl–iso–butyl phthalate causing suicidal germination of broomrapes seeds, it can be exploited as an effective and helpful tool in broomrape management programs in faba bean fields. Genotype; Orobanche spp; Parasitic weeds; Suicidal germination; Vicia faba
显示更多 [+] 显示较少 [-]Genotype × environment interaction and stability analysis for some economic characters in snap bean
2019
Noura Taha
Twenty-one new promising lines in addition to four standard cultivars of snap bean (Phaseolus vulgaris L.) were evaluated under eight environmental conditions (four sowing dates in two seasons of 2015/2016 and 2016/2017). A randomized complete block design (RCBD) with three replicates was used for each sowing date at Barrage Horticulture Research Station (BHRS), Qalubia Governorate, Horticulture Research Institute (HRI), Agricultural Research Center (ARC), Egypt. Data were recorded for some traits i.e., number of days to flowering, pod length, pod diameter, fiber content, total green yield and rust disease severity. The results showed that the linear response of environments was highly significant for all studied characters, indicating the differences between environmental conditions and their considerable influence on these traits. Mean squares of genotypes (G) were highly significant for all studied traits, suggesting the presence of wide range of differences between studied genotypes concerning all investigated traits. Mean squares of the other main sources of variation, i.e., years (Y) and sowing dates (D) were also highly significant for all traits except year for total green yield/feddan trait. The mean square due to environment + (Genotypes × Environment) was significant for all studied traits, indicating that there was a considerable interaction of genotypes with environmental condition in different eight environments. Significance of Genotypes × Environment (linear), reveals that genotypes differed in their considerably across different environments. The linear response of environments was highly significant for total green yield trait, indicating that genotypes differed in their regression on the environmental index. The results of stability analysis conclude that the six new lines G1, G2, G3, G7, G10 and G18, were the most stable genotypes, which gave the maximum total green yield overall the eight studied environments and were adapted to environments for total green yield. It is evident that these genotypes could be used as stress tolerant genotypes under stressed environments.
显示更多 [+] 显示较少 [-]DEVELOPMENT AND EVALUATION OF NOODLES PREPARED FROM RICE AND WHEAT FLOUR BLENDS
2019
Maghfera abd-elfatah | Mamdouh El kalyoubi | Ihab Ashosh | Mohamed Mostafa
Noodles were prepared by incorporation varying blends (0, 20, 40, 60 and 80%) of rice flour in replacement of wheat flour. Noodle samples were evaluated for their proximate composition, scavenging activity, sensory properties and cooking quality characteristics. The proximate composition of uncooked noodles varied significantly, with increasing rice flour level that replaced wheat flour , a gradual decreases in moisture , ash , crude fiber and protein contents in noodle pastes were occurred , their values ranged between 4.02-5.33% ; 1.11-5.22% , 0.65-0.82% and 8.51-12.81% ,respectively. However, an increases in fats, total carbohydrates and energy value were take place ranging between 4.62-8.91 % , 71.11-76.74% and 377.64 - 421.43 kcal/100g, respectively .The antioxidant activity ranged between 22.2% for wheat based noodle (100% wheat flour) to 36.8% for sample containing 80% rice flour as scavenging activity for free radicals. The overall acceptability of wheat-based noodles (WF: RF 100:00) were rated superior for their sensory preferable characteristics. Among the blended flour noodles, the noodles incorporated with up to 40% rice flour received the same acceptability as wheat-based noodle. The cooking quality characteristics differed significantly among the noodle samples; the cooking time ranged from 6.5 to 10.3 min, it was more in wheat-based noodles than noodles from rice flour. Cooking loss; water absorption percent; cooked weight and swelling index values ranged from 0.1 to 0.6%, 137.1 to 231.6%, 23.74 to 33.24 g/g and 3.20 to 5.38 %, respectively; which revealed that a significant hindered was occurred in the functional paste properties and cooking quality parameters with increasing the level rice flour in noodles. The study indicated that, noodles prepared from mixtures of wheat and rice flour showed that the best acceptable sensory attributes and cooking quality characteristics were observed at blending ratio 40 : 60% rice flour : wheat flour . These findings enhance the utilization of non- conventional flours like rice flour for the development of value added noodles with reduced gluten content and boost nutritional value.
显示更多 [+] 显示较少 [-]Effect of vitamin E-selenium supplementation on some semen quality traits of Muscovy drakes.
2019
Safaa Mostafa | Ibrahim Elsyed | Ayman Hassan | Amal Hassan
A total of 12, 36-week-old of Muscovy drakes were divided randomly into three equal groups (4 individuals each) and subjected to supplementation of VE-Se in combine form in drinking water. The first group was served as control group (C), while the second and third groups (T1 and T2) were supplemented with 1.0g/l and 1.5g/l of VE-Se in drinking water, respectively. The study was performed at poultry research station which belongs to department of poultry production, faculty of agricultural, Ain Shams University and lasted 30-dayes period during summer season. Semen quality traits in terms of ejaculate volume (ml), spermatozoa concentration (×109), sperm motility (%), dead and live (%), PH value and abnormal sperms (%) were determined. Sperm reserves in ducts, seminiferous tubules diameter (STD) and relative spermatogenic activity were also studied. The obtained results indicated that addition of vitamin E-Se improved ejaculate volume, sperm concentration and motility score, while reduced the percentage of abnormalities and dead sperms. Results showed that semen ejaculate volume was significantly increased (P<0.05) with increasing the level of VE-Se supplementation in drinking water. Spermatozoa concentration, progressive motility (%), and packed sperm volume were increased significantly with VE-Se treatment. The percentages of dead and abnormal spermatozoa were significantly decreased (P<0.01) in VE-Se treated compared with the control drakes. Concerning with the effect of two levels (T1 and T2) of VE-Se supplementation in drinking water of Muscovy drakes on the morphometric traits of testis, the obtained results showed that highly significant effects of antioxidant (VE-Se) supplementation on the morphometric of seminiferous tubules (p < 0.05) and the higher values of sperm reserves and spermatogenic activity percentage in both T1 (4.4/ml×109 and 82.8%) and T2 (4.5/ml×109 and 83.0%) compared with control group (3.2/ml×109 and 61.3%). We conclude that VE-Se supplementation could lead to greater economic benefits through increased reproductive efficiency within the ducks production industry.
显示更多 [+] 显示较少 [-]Influence of Different Nitrogen Sources on Growth and Pathogenic Capability of Rhizoctonia solani Causing Root Rot of Faba Bean
2019
Maha Mohamed
ABSTRACT The effect of different nitrogen sources (glucosamine sulfate, ammonium sulfate, aspartic acid, phenylalanine and peptone) in comparison to sodium nitrate, the major nitrogen compound in basal agar Czapek’s synthetic medium growth were studied on the linear growth of Rhizoctonia solani and its pathogenicity on faba bean germinated seeds. Ammonium sulfate exhibited faster liner growth and showed the same effect as the basal medium with sodium nitrate while, glucosamine sulfate showed less growth rate compared with sodium nitrate. Glucosamine sulfate and ammonium sulfate showed significant reduction in number of infection cushions which led to significant decrease of disease index in vitro. Our results showed strongly significant positive correlations between disease index and both of diameters of hyphae and number of infection cushions while, very weak correlation coefficient was found between disease index and linear growth of R. solani. Under greenhouse conditions, glucosamine sulfate or peptone as a sole nitrogen sources in food requirements of Rhizoctonia solani inoculum depressed the virulence of the fungus. The effect of different amounts of glucosamine sulfate was determined on fungal growth rate, infection cushions, disease index in vitro and polyphenol oxidase activity. Increasing amount of glucosamine sulfate showed significantly reduction of growth rate in comparison to the basal medium with sodium nitrate. All seeds subjected to R. solani grown on different amount of glucosamine sulfate showed the lower number of infection cushions, disease index and polyphenol oxidase activity compared with sodium nitrate. Under greenhouse conditions, disease index showed significantly decreased effect when glucosamine sulfate used as soil applications and showed better effect on shoot weight and root weight compared with control plants treated with sodium nitrate. Our study proposes that glucosamine sulfate may acts as controlling factor of pathogenicity genes of R. solani. Key words: Rhizoctonia solani, faba bean, Vicia faba, nitrogen sources, glucosamine sulfate, pathogenicity.
显示更多 [+] 显示较少 [-]Isolation and identification of halophilic bacteria producing exopolysaccharides from whey and milk permeate
2019
Amal Hegazy | Mohamed El-Nawawy | Ali Ali | Yehia El-Samragy
The aim of this research was to utilization of the salt whey and milk permeate to produce exopolysaccharides (EPSs). Where cheese whey is simultaneously an effluent with nutritional value and a strong organic and saline content. However, it is drained in the sewers, the EPSs are highly heterogeneous polymers produced by different species of bacteria and have recently been attracting considerable attention from biotechnologists because of their potential applications in many fields. Thus, we have isolated some halophilic bacteria that showed the ability to produce EPS from whey and milk permeate. A total of 46 strains of moderately halophilic bacteria were isolated from two types of samples. The first type was dairy samples (Baramily cheese whey and mish cheese), while the second type was water samples (salty lake water and its sediments) of Wadi El-Natrun Valley (lakes Hamra, UmRisha, and Baida), Beheira Governorate, Egypt. From isolated strains there are twelve strains were having the ability to produce exopolysaccharides but only seven strains can produce EPS from whey and milk permeate. The growth conditions i.e. concentrations of NaCl, pH value and different incubation temperature, of isolates were determined. The effect of these conditions on the production of EPS was investigated. The obtained results indicated that the optimum conditions for the production of EPS by these strains were 10 % NaCl, pH 7 and the optimum incubation temperature was 37°C. Three strains showed the highest production of exopolysaccharides. These strains were identified using two methods the first method was biology system and the second one was 16S rRNA sequence analysis method. It could be identified as Alteribacillus bidgolensis and Bacillus licheniformis. Alteribacillus bidgolensis (strain P4B) produced the highest amount of EPS (52 g/L) from whey followed by Bacillus licheniformis (DSM 13) (42 g/L), while the highest amount of EPS produced from milk permeate was (43 g/L) by Alteribacillus bidgolensis (strain P4B) followed by Bacillus licheniformis (DSM 13) (36 g/L).
显示更多 [+] 显示较少 [-]Interaction between Biotic and Abiotic Agents to Control of Potato Bacterial Wilt Disease
2019
Hanan Abdrabouh | Afaf Zein El-abdeen | Nagy Abdel-Ghafar
Bacterial wilt disease caused by Ralstonia solanacearum (Smith) Yabuuchi, et al. (1995) is one of the most important bacterial diseases over the world. This study was planned to control the disease using interaction between biotic and abiotic agents, under greenhouse experiment. In vitro, tested bactericides (gentamycin, ceflaxcin and copper sulphate) were decreased growth of R. solanacerum compared with control treatment. Copper sulphate was the most effective compared with ceflaxcin and gentamycin, but ceflaxcin was moderately effective and gentamycin was less effective against the pathogen. Inhibition zone diameter was increased with increasing concentrations of tested bactericides. Application of different bio-agent isolates (Pseudomonas fluorescence, Bacillus subtitlis, Bacillus megaterium and Serratia mareacescne) were reduced the growth of the pathogen compared with control treatment. B. subtilis isolate was the most effective, but isolates of Ps. fluorescens and B. megaterium were moderately effective and S. marescences isolate was less effective against the pathogen. Greenhouse experiments, disease severity were decreased and potato yield were increased with interaction between bio-agents (Bacillus subtilis, and Pseudomonas fluorescence isolates) and bactericides (gentamycin and ceflaxcin), or bio-agents and resistance inducers factors (salicylic acid and jasmonic acid) or bactericides and resistance inducers factors compared with control treatment. However, interaction between bio-agents as tuber treatment and bactericides as soil drench treatment or interactions between bactericides as soil drench treatment and resistance inducers as foliar treatment were the most effective against the disease and the yield. But, interactions between bio-agents as soil drench treatment and resistance inducers as foliar treatment were moderately effective to control the disease and the yield. But, interaction between ceflaxcin as bactericide and Ps. fluorescens isolate as bio-agent or salicylic acid as resistance inducer were the most effective on disease severity and the yield compared with other treatments, but interaction between B. subtilis isolate as bio-agent and jasmonic acid as resistance inducer were less effective compared with other treatment.
显示更多 [+] 显示较少 [-]ANTIBACTERIAL ACTIVITY OF GARLIC EXTRACT AND DEHYDRATED GARLIC POWDER AGAINST LISTERIA monocytogenes IN SYNTHETIC MEDIUM AND SOFT CHEESE
2018
R.R. Al-Hindi, | El-Ghani Abd
DETECTION OF MUTAGENICITY IN SOME CURED MEAT PRODUCTS USING AMES TEST
2018
Kariman Hassan | R. Mahmmod | M. Khallaf | Lamyaa Sayed
The use of food additives must be under control specially with the improvement of many diseases such as cancer disease which became the most threaten disease all over the world, although there had become more health aware and medical culture, many unhealthy food products are being consumed increasely, so it became very important to study some food product’s mutagenicity. Detecting mutagenicity with short term assay with high percentage sensitivity are specifications available at Ames test with the mutated Salmonella typhimiurium strains and the reverse growth of the mutated bacteria was an indicator to the sample tested mutagenicity .The aim of this study is to evaluate the effect of adding sodium nitrite salt with various levels on mutagenicity in two of processed meat products (pastirma and luncheon) using Ames test. The results gave in the tested samples sign of mutagenicity at low concentrations and high reverse growth at higher concentrations , sodium nitrite extract gave highest mutagenicity at 10% (1.5 ml dose ) concentration , pastirma extract gave highest mutagenicity at 10% concentration (2 ml dose ) and luncheon extract gave highest mutagenicity at 100% concentration.
显示更多 [+] 显示较少 [-]STUDIES ON GRAFTING AND SOME FOLIAR SPRAY TREATMENTS ON WATERMELON PRODUCTIVITY UNDER NORTH SINAI CONDITIONS
2018
M. Wehedy | M. Hafez | I. El-Oksh | A Abou Elyazied
The influences of grafting treatments (6001 and Star rootstocks in addition to, check nongrafted transplants) and some foliar spray treatments (potassium silicate at rate 4 and 5 ml/L, calcium at rate 1 and 2 ml/L and check) on growth, yield and fruit quality of watermelon cv. Aswan F1 were investigated. The experiment was conducted in Baloza Research Station, Desert Research Center, at North Sinai Governorate during the two successive growing seasons of 2016 and 2017. The results indicated that plants sprayed with potassium silicate at a rate 5 ml/L or grafted onto Star rootstock recorded the highest significant values on transplant stand (success) percentage, vegetative growth characteristics (plant length, fresh and dry weight), yield and its components (fruit weight, fruit size, rind and flesh thickness and total yield), while the lowest values were observed in check nongrafted plants. Meanwhile, grafting had reducing effect on fruit quality, where the highest values of total soluble solids and total sugars were found in the fruits of nongrafted plants. The application of 5 ml/L potassium silicate gave the best significant values of TSS and total sugars compared with check treatment during the two studied seasons.
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