Tamarix nilotica is a perennial halophyte growing naturally at the Mediterranean coastal salt marshes. Vegetative parts were collected in two successive winters and summers to investigate the seasonal effects on the ecomorphological and physiological responses of T. nilotica. The results indicated that either in winter or summer seasons, T. nilotica can reduce the effect of soil salinity by excreting salts outside its body through salt glands. Summer season was characterized by low content of soil moisture (due to rare rainfall), high soil EC, high light intensity and high temperature; there-fore, plant induced certain morphoanatomical changes in leaves and stem to face the previously mentioned adverse conditions. The most remarkable changes to reduce transpiration process was found by decreasing leaf area and increasing cuticle thickness and mesophyll tissue thickness. In addition, the most marked physiological changes in summer were the significant in-crease in total phenols, proline, free amino acids and total soluble sugars. These compounds can work as osmotic regulators and/or antioxidants. These features enhance the defensive mechanism against dehydration and permit T. nilotica to tolerate the stress conditions in salt marsh habitat.

In contrast to the global countries, ectomycorrhizae members are not common in Egypt, however, they are very important in the afforestation of poor lands. Their occurrence in Pinus sp., Clerodendrum sp., and Eucalyptus sp. may help to explain why these trees are the most extensively dominant around the world, especially in Egypt. The identification and characterization of numerous ectomycorrhizal fungi often involve large morphological descriptions of sporocarps alone, which consequently, in some instances, raise arguments about the accuracy of these studies. The present work was achieved to isolate, identify, and characterize three ectomycorrhizal fungi from sporocarps combining morphological and molecular techniques. The morphological characteristics of tested species were assessed and compared to standard taxonomic literature. ITS-rDNA was utilized for molecular analysis using the universal fungal primers ITS1. Identification of these species was confirmed by comparing the sequences of amplified genomes of these species with respective species sequences in GenBank, followed by blast analysis.

The main objectives of this study were to use rye grain in the diet of Turkish sheep as a substitute for barley grain and to understand the bio-logical value of rye in feeding lambs and weight gain. Animals were divided into 3 experimental groups with 3 replicates in each group. Control group (T1) received standard diet with barely grain while groups T2 and T3 fed on diets with substitution of 20 and 30% barely grain by rye grain. The results of the study showed that lambs fed on 20% rye seeds have a significant high-er weight gain as a results of better feed intake and feed conversion.

The study investigated the influence of traditional nitrogen, phosphorus and potassium (NPK) mineral fertilizers and nano-fertilizers on yield, and its attributes, and fiber characters of two Egyptian cotton varieties Giza 94 and Giza 96. The design of the experiment was a split- plot with four replicates. Results indicated that Giza 94 cultivar surpassed Giza 96 in agronomic characteristics, although Giza 96 exhibited the highest fiber reading. Nano-chitosan (NPK)-fertilization significantly improved the mean value of boll weight, seed yield, lint yield, lint percentage, upper half mean (UHM), fiber uniformity index, fiber tenacity, and Micronaire value in both seasons. Conversely, the most minimal values for the aforementioned characteristics were recorded with the control NPK fertilizer over the two seasons.

The combined data from two growing seasons in a field experiment study by using two irrigation systems (surface and subsurface drip) in terms of additional water for sugar beet plants under saline conditions are used to estimate root and sugar yield, water use efficiency and root penetration power at the time of harvest. Water salinity levels of 6000 and 8000 ppm were applied to irrigate sugar beet from the time of planting on October 3 to the time of harvest on April 22 with three additions of water (limited 1750, moderate 2500 and optimum 3250 m3/fed). The results are summarized as follows: The heaviest root and highest sugar yield as well as highest water use efficiency were recorded when using subsurface irrigation system, 6000 ppm water salinity level, and moderate or optimum irrigation water (2500 or 3250 m3/fed). Oppositely, the highest penetration power values were obtained using drip irrigation systems with low water quantity (1750 m3/fed) and high-water salinity level (8000 ppm).

The importance of water footprint (WFP) is providing information related to water resource management, especially for countries that have water scarcity and rely on irrigation to enhance food security. A field experiment was conducted during two winter seasons in 2018, and 2019. The current study sought to evaluate the impacts of various nitrogen levels (1.N1: 120, 2.N2: 150, and 3.N3: 180 kg /fed) on potato production as well as the water footprint and water requirement. Vegetative characteristics, yield parameters, N contents, and climate data were measured. The obtained results are clarified that increasing the nitrogen rate up to 180 kg/fed led to increase the vegetative growth characteristics, yield parameters, and water footprint of potato crop. The most significant vegetative growth values were obtained using a 180 kg/fed nitrogen level followed by 150 kg/fed. The greater marketable tuber yield was recorded at 180 kg/fed with 18117 & 17753 Kg/fed values, followed by 150 kg/fed with 16864 and 16545 Kg/fed values for the first and second seasons respectively. The water footprint of potato in Egypt ranges from 237 to 267.8 for nitrogen levels of 120 kg /fed and 180 kg /fed.

This study was conducted to evaluate the effect of salt stress on amino acid profile and proximate analysis of fenugreek germination samples of two varieties: Giza 2 and Giza 30 for three days. The germination of sterilized fenugreek seeds was conducted using tap water and NaCl solution (2,000 ppm) where higher salt concentration lowered fenugreek germination quality. The germination of fenugreek seeds using tap water or brine increased the crude protein in both dry Giza 2 and Giza 30 seed varieties. Furthermore, sedative sprouts of Giza 30 recorded the highest crude protein (30.60%), fat (6.20%) and energy (333.13 kcal/g) while the use of sterilized Giza 2 seed variety recorded a higher percentage of carbohydrates (43.3%), fiber (8.10%) and ash (6.60%). Alternatively, although amino acid profiles indicated that aspartic acid and proline were most abundant in fenugreek sprouts, sulfur amino acids (cysteine and methionine) demonstrated the least values of the two varieties. Adopting saline water in germinating fenugreek seeds for the two Giza 2 and Giza 30 varieties decreased all amino acid contents compared with those of tap water treatment.

This study evaluated the use of biologically treated olive mill wastewater (OMWW) for irrigation of pea plants, rather than discharging this nutrient-rich liquid and polluting the environment. Pea seeds were planted in pots containing soil irrigated with tap water (control), untreated (crude) OMWW, or OMWW treated with the fungus, Pleurotus columbinus, or algae Spirulina platensis or Wollea sp., with two NPK rates. Plant length shoot and root dry weight, nitrogen, phosphorus, potassium, chlorophyll, and carotene contents were measured, along with to nitrogenase and dehydrogenase activity. The highest shoots N and P content were recorded in plants irrigated with Sp. Platensis-treated OMWW + 100% NPK; while the highest K content was in plants irrigated with crude OMWW + 100% NPK. The highest dehydrogenase activity, 59.01 μg TPF/100 g soils, was recorded in plants irrigated with P. columbinus-treated OMWW supported with 75% NPK, while maximum nitrogenase activity (261.82 μmol/100g soil/day) occurred in plants irrigated with Wollea sp.-treated OMWW with 75% NPK. The highest content of chlorophylls a & b and carotene (0.838, 0.276, 0.252 mg/g dry weight, respectively) were found in plants irrigated with OMWW treated with Wollea sp. and 100% NPK. Thus, biologically treated OMWW showed promising impacts on plant growth parameters.

Pink-pigmented facultative methylotrophic bacteria (PPFM) isolated from cotton leaves was identified based on phenotypic and genotypic characteristics as Methylobacterium rodiotolerance. Two field experiments were conducted to investigate the effects of PPFM, methanol (10 and 30%) and a combination of bacteria and methanol on the growth, fruit quality, and yield of two strawberry cultivars (Florida and Festival). The main differences between the two cultivars are greater foliage fresh weight and early yield in cv. Florida while Festival cv. had a higher total yield per plant and greater anthocyanins and ascorbic acid contents. The greatest vegetative growth, dry matter percentage, potassium content, and carbohydrate content besides the earliest yield per plant were observed following spraying with PPFM or PPFM mixed with 10% methanol. Spraying with PPFM resulted in the highest total yield per plant, highest yield per feddan, and fruit quality. Spraying cv. Florida with PPFM resulted in the best early yield while spraying cv. Festival with PPFM resulted in the highest total yield and fruit quality. Spraying with PPFM appears to be the most efficient treatment for enhancing the total yield of Festival cv. by an average of 23.02 and 24.06 tons per feddan in the first and second seasons, respectively.

In order to reduce the use of chemical fertilizers, the use of biological inoculants has been increased. Beneficial microorganisms are used to increase crop yields by stimulating plant growth through the production of phytohormones. In the present study, Indole Acetic Acid (IAA) production was analyzed in 18 bacterial isolates with Plant Growth-Promoting (PGP) capabilities; 15 of these isolates were Rhizobium, and three were Serratia. All isolates have been characterized morphologically and biochemically, and their IAA production in the presence of tryptophan, a precursor for IAA biosynthesis, has been evaluated. IAA production was detected using the Salkowski reagent with CT-2200 spectrophotometer at 530 nm. The levels of IAA production varied between the different isolates. The top two IAA producers were selected for genetic identification using 16SrRNA primers (27F and 1492R). One of the Rhizobium isolates (NRC-R2) shared 95% sequence similarity with Rhizobium sp., according to a Blastn search in GenBank and Rhizobium leguminosarum, whereas the Serratia isolate (Ain Shams Center for Genetic Engineering and Biotechnology (ACGEB)-S2 was 99.5% similar to Serratia sp. In the near future, it is possible that these two isolates could serve as biological sources for IAA.