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DNA Barcoding of Commercial Three High Value Red Sea Fishes from The Egyptian Market 全文
2021
rana gamaleldin | Mohamed Rashed | Mahmoud Magdy
Fish play an essential role in food security and are regarded as one of Egypt's primary food resources and, therefore, significantly im-pact the national economy. The three main sources of fish production in Egypt: marine (Red and Mediterranean seas), lakes and the River Nile, due to the elevated incidence of species substitution at the global level, precise identification of seafood species on the mar-kets considered a solution for food safety con-trol institutions and human consumer protec-tion. Mislabeling occurs when one species is substituted for another. Different species of red sea marine fish were collected from the Egyp-tian market. According to the Arabic name in the fish market, Samples searched for English and Latin family names in (www.fishdata-base.org). Commercial samples were success-fully extracted and confirmed with 1.5% aga-rose gel electrophoresis. The result showed that the mtDNA gene cytochrome c oxidase subunit I (COI) amplification was successful for all samples with high concentration, it was purified and sequenced for both directions and blasted using the NCBI database. Resulting in the identification of three commercially essen-tial and expensive fish samples, Baghbaghan (parrotfish), Hamour (Greasy Grouper) and kahaya (Spiny squirrelfish). One of them cor-rectly identified according to the market name, while the other two are recorded mistakenly under another name.
显示更多 [+] 显示较少 [-]Evaluation of Some Transpiration Regulators in Sunflower for Saving Irrigation Water in Relation to Productivity 全文
2021
Naglaa Taha | Ayman Abohadeed | Taher Fayed | mohamed fergany
An experimental field trial was conducted in seasons of 2015 and 2016 at Giza Experimental Station of Agricultural Research Centre (ARC), Egypt to evaluate the foliar spray effect with transpiration regulators (Potassium, Kaolin and Ascorbic acid) and three levels of irrigations (100%, 80% and 60% of Et0) on yield, its attributes, and water productivity of two sunflower cultivars (Giza 102 and Solala 120). The factorial treatments were arranged in split- split plot design with three replications. The irrigation levels (100%, 80% and 60% of Et0) were devoted for main plots, while subplots contained the three spraying treatments of transpiration regulators. (Potassium at 1%, Kaolin at 3% and Ascorbic acid at 100 ppm), in addition to control treatment (water spray). Sub-sub plots were occupied by the two sunflower cultivars (Giza 102 and Solala 120). Obtained results proved that, increasing irrigation levels up to (80- 100%) caused significant increase in all growth characteristics, photosynthetic pigments (total chlorophyll), relative water content (RWC %) and seed oil %. Also, yield parameters being head diameter, 1000-seed weight and seed yield) show significant reduction under water deficit. Foliar spray with (Potassium at 1%, Kaolin at 3% and Ascorbic acid at 100 ppm), positively affected all the growth and physiological criteria of the tested plants compared with control. Generally, under low irrigation levels, application of transpiration regulators effectively reduced the detrimental impact of drought stress on growth, yield and its components of the two used cultivars in the two seasons. While, foliar spraying of potassium under 100% of Et0 gave the best treatment in this respect. Concerning cultivars, WUE (water use efficiency) for Solala 120 was significantly higher than for Giza 102 in the two seasons. All interactions among factors under study due to WUE were not significant in 2015 and 2016 seasons.
显示更多 [+] 显示较少 [-]Using Probiotic Bacteria for Soymilk Fermentation 全文
2021
Abdallah Gad | Mona Orabi | Dina Abdelghani | Khadiga Aboutaleb | Shimaa amin
Five probiotic bacterial strains (Lactobacillus plantarumATCC 14917,Lactobacillus caseiDSM 20011, Lactobacillus acidophilus ATCC20552, Lactococcus thermophilus DSM 20259,and Bifidobacterium longum B41409) were used as monoculture, and combined with them as co-cultures for the fermentation of soymilk. The total number is 20 co-cultures, separated similarly into two parts, the first half of each co-culture consisted of two strains, and the second half of each co-culture consists of three strains. The findings revealed that these cultures were capable of fermenting soymilk at only 8 h with a pH drop of between 4.42 and 4.89. Among 25 cultures, eight cultures (3 monoculture of L. plantarumATCC 14917 (C1),Lc. thermophilusDSM 20259 (C4), and B. longum B41409 (C5) and co-cultures of two strains L. plantarum ATCC 14917+ Lc. thermophiles DSM 20259 (C8) and of three strains L. plantarum ATCC 14917 + Lc. thermophilus DSM 20259 + B. longum B41409 (C18), L. plantarum ATCC 14917+ L. caseiDSM 20011+ Lc. thermophilus DSM 20259 (C19), L. caseiDSM 20011+ L. acidophilus ATCC20552+ Lc. thermophilus DSM 20259 (C22), and L. acidophilus ATCC20552+ Lc. thermophilus DSM 20259 + B. longum B41409 (C25)) which recorded the greatest significant viability of bacterial cells, titratable acidity, total organic acids (lactic, acetic, and propionic acids) contented, and pH in fermented soymilk ranged from 9.26 to 9.98 Log CFU/mL, 3.06 to 3.32 %, 19.90 to 18.40 g/L (lactic acid), 13.30 to 12.30 g/L (acetic acid), and 16.35 to15.00 g/L (propionic acid), and 4.38 to 4.62. The soy yogurt produced from the selected fermentation of soymilk was more preferred in the chemical composition of protein, carbohydrate, and fat contents than non-fermented soymilk.So the results show that the integrative use of probiotics may achieve the nutritional value of soymilk after fermentation had been improved by probiotic strains, therefore, making it a more health-beneficial functional food.
显示更多 [+] 显示较少 [-]Nitrogen Nutrition and Adaptation of Halophyte Chenopodium quinoa to Salt Stress 全文
2021
Karema Tarek | Ibrahim Alshamy | Sayed Hussin | Sayed Eisa
There is little evidence that the nitrogen nu-trition supply at rates above or less than what is considered optimal in non-saline conditions improves growth and yield of halophyte crop cultivated under salt stress. Therefore, hypoth-esize of the present work was to find out the magnitude to which N could restore the harm-ful effects of salt stress on quinoa plants. A pot experiment was performed in greenhouse con-ditions to evaluate quinoa's response grown under water salinity treatments (0.0 & 200 mM NaCl) when nitrogen nutrition rates were limiting (50ppm), adequate (250 ppm), and ex-cess (450 ppm) to guide proper application rate of nitrogen fertilizer under salinity stress. The results indicated that, salinity caused a signifi-cant decrease in the vegetative growth of the plant. Consequently, all vegetative measure-ments were negatively affected. As a result, the seed yield decreased to more than 50%. The application of a moderate level of nitrogen (250 ppm) caused a significant ameliorative effect on seed yield by 126% under non saline conditions and 34.5 % under saline conditions compared to the low nitrogen level. The results did not improve any further with the applica-tion of a higher level of nitrogen. These results indicate that applying (N) in adequate may im-prove most traits and prove to be a physiolog-ical treatment to increase resistance against the negative effects of salt stress in quinoa.
显示更多 [+] 显示较少 [-]Foliar Fertilization of Different Species of Mulberry Trees and Its Impact on Silkworm Bombyx mori Productivity from Cocoons and Eggs 全文
2021
sawsan abdelmegeed
Rearing mulberry silkworm was found to be dependent on leaves quality of mulberry trees, larvae of mulberry silkworm fed on two species of mulberry trees Morus alba and Mo-rus nigra were treated with foliar fertilizer (Basfoliar 20-19-19 SP contains 20% N, 19% P2O5, 19% K2O, 5% NO3, 3.6% NH4 and 11.4% NH2) with three different concentra-tions,1%, 3% and 6% in addition to control treatment for comparison. When larvae were fed on leaves of mulberry trees one day after Treatment, high mortality (up to 100%) was observed specially with 6% concentration. Mean weights of larvae, cocoons and cocoon shell and fecundity were negatively affected when compared to control. On the other hand, when larvae were fed on leaves of mulberry trees, no mortality occurred after two weeks of treatment in all concentrations, in addition, the mean weights of larvae at the end of 4th and 5th instar larvae, cocoons and cocoon shell s in-creased at 3% concentration compared with other concentrations and control. The highest fecundity occurred in female moths when lar-vae were fed on leaves of M .nigra compared with M. alba as well as 3% concentration com-pared with other concentrations and control.
显示更多 [+] 显示较少 [-]Potentiality of Using Mycorrhizae and Pseudomonas fluorescens in Reducing the Effect of Water Shortage on Broccoli Plants 全文
2021
Dalia Abd El-Fattah | Fadl Hashem | Ahmed Farag
Accelerating global warming and water scarcity and improving water use efficiency are considered essential factors for achieving adequate crop development and productivity. Therefore, the authors targeted the use of arbuscular mycorrhizal fungi (AMF) and Pseudomonas fluorescens (Ps1) for improving growth, productivity, and water use efficiency of broccoli plants (Brassica olercea L. var. italica, cv. Belstar F1) under various irrigation regimes i.e., 50, 75, and 100% of crop evapotranspiration (ETc). Two greenhouse experiments were carried out in clayey soil. The combined inoculation of endomycorrhizae and Ps. fluorescens (Ps1) improved water use efficiency and consequently vegetative growth and yield. Under the applied irrigation regime 75% ETc of broccoli inoculated with both endomycorrhizae and Ps. fluorescens (Ps1) showed higher head weight (616 and 647 gram) than those grown under the irrigation regime 75% ETc combined with endomycorrhizae (568 and 559 gram) during the two seasons, respectively. However, a minimum yield value of 149 and 142 grams per plant was recorded for un-inoculated plants grown under 50% irrigation regime during both seasons. In conclusion, the combined inoculation with endomycorrhizae and Ps. fluorescens (Ps1) under irrigation regime 75% ETc was the optimum combination for increasing water stress resistance and broccoli productivity under water scar-city circumstances.
显示更多 [+] 显示较少 [-]Effect of Clinoptilolite on Humoral Immunity and Biochemical Parameters in Calves Vaccinated with Foot and Mouth Disease Vaccine 全文
2021
Nour Elhoda Tamim | Dawlat Salama | Hani Srour | Ehab Ibrahim
The foot and mouth disease (FMD) vaccine is used to control the FMD in cloven-hoofed animals including calves. The most important step in vaccine preparation is the selection of a suitable adjuvant which plays a main role in the vaccine’s potency. The present study is an attempt to use clinoptilolite instead of Montanide oil to enhance the efficiency of the FMD vaccine and reduce its production cost. Twelve calves were split into four groups. Three groups were vaccinated with FMD vaccine with different adjuvants i.e. 100% Montanide (ISA 206), 50% of Montanide with 1.0 μg/dose of clinoptilolite (ISA 206+CLINP), and by replacing the 100% of Montanide to 1μg/dose of clinoptilolite (CLINP). The fourth group was unvaccinated. Results showed that calves vaccinated with (ISA 206+CLINP) FMD vaccine expressed the highest and the longest periods of immune response until 38 weeks. While groups vaccinated with (CLINP) or (ISA 206) vaccine showed only protection up to 26 and 34 weeks respectively. Also, our results showed that clinoptilolite improved organ functions resulting from the stress of vaccines. Finally, this study recommended the use of clinoptilolite with Montanide oil as a potential adjuvant in the FMD vaccine to give high stimulation of the immune response.
显示更多 [+] 显示较少 [-]Susceptibility of Some Ornamental Plants to Aphid Infestation and Effects on Plant Biochemical Components and Morphological Structures 全文
2021
Rania Rashwan
Aphid is a major insect pest attacking ornamental plants. It causes great damage by reducing the economic values of plants. The investigated research aims to determine the relation between leaf biochemical components, morphological structures of plant leaf surface and the susceptibility of seven ornamental plants to Aphid Infestation. Highest recorded aphid infestation was observed on Tecoma stans during 2017 and 2018 seasons followed by Rosa damascena and Jasminum grandiflorum. There was no rec-orded infestation on four inspected ornamental plants, Ruta graveolens, Schefflera actinophylla, Nerium oleander and Bougainvillea spectabilis. Activity of antioxidant enzymes plays as defense line when increasing the insect population density. Biochemical components are varied among different plants that prevents or reduces insect infestation, as tannins, total phenol, and Phenol oxidase. Trichomes are considered as main morphological character in plant defense system. These results enhanced utilization resistant ornamental plants due to preventing aphid infestation. It is considered one of the integrated pest management programs, that achieves health environment without more insecticidal application.
显示更多 [+] 显示较少 [-]Preparation and Thermal Stability of α-Tocopheryl acetate and Strawberry Anthocyanins Complexed with Starch and β-Cyclodextrin 全文
2021
Eman Rashed | Hussein Ali | Mohamed Attia | Eslam Bendary | Khaled Ramadan
In the sake of enhancing the thermal stability of α-Tocopheryl acetate (α-TA) and Strawberry Anthocyanins (AN), their encapsulation with starch or β-Cyclodextrin were prepared and characterized by UV-Vis and IR spectra in addition to thermal analysis. Thermal stability was investigated by comparing the thermographs of each host and guest with that of the prepared complex. Both hosts provided extra thermal stability to both guests where α-TA is stabilized from 230 to 291oC by complexation with starch and to 285oC by encapsulation with β-CD. AN was also stabilized from 40.02oC to 75.57 and 79.46oC by complexation with starch and β-CD respectively.
显示更多 [+] 显示较少 [-]Phylogeny Validation for Some Egyptian Brassicaceae Endemic Species 全文
2021
Shaimaa Abd Elhady | Abdoallah Sharaf | Aly Salam | Khaled Abd El- Atey | Asmaa Abu Shady
A crucial menace for the biodiversity in arid and semi-arid territories is the global warming arising from anthropogenic activity. Egypt is expected to undergo an acute rainfall decrease and temperature boost in the next few decades, leading to many plant species' geographical allocation. Endemic plants of pleiotropic economic importance are strongly affected by climate change prospects, which will gradually result in losing our plant wealth genetic resources. Due to few studies on the Egyptian Brassicaceae family which has an economically and medicinally importance due to the presence of many active compounds that are included, in the pharmaceutical and cosmetic components (e.g., glucosinolates) besides having a large amount of antioxidant which inhibit the growth of microbes and also treat rheumatic diseases. The identification of this family still kind of fishy as researchers mostly relies on the morphological characters. To globally sustain this Egyptian plant family wealth's genetic pattern, it is substantial to characterize them based on their authenticated genetic background. Here we present a phylogenetic analysis of 16 species of the Egyptian Brassicaceae family using two plastid coding genes; Ribulose-1,5- bisphosphate carboxylase oxygenase (rbcL-a) and maturase K (matK). The maximum likelihood of the two markers for our samples was concordant with the Brassicaceae's references-sequences, which exist on plastid are considered highly conserved biomarkers. In conclusion, we have generated a robust phylogeny tree based on the molecular level that validates the Egyptian plant species and reliably differentiates them on morphological identification. This study is considered the first phase of Egyptian Brassicaceae family species authentication followed by biochemical studies serving the pharmacological and medicinal fields
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