The present study aimed to evaluate the effect of dietary nucleoforce® supplementation on the antioxidative status and the molecular expression of IGF-1, IL-1ß and IL-6 genes in broiler chickens. 240, one-day old Ross 308 broiler chicks were randomly allocated into four equal groups, each of which contained 60 birds and was divided into three replicates with 20 birds for each replicate. Birds in the first group fed basal diet and considered as a control group (C), whereas birds of the second (200N), third (350N), and fourth (500N) groups fed diet supplemented with 200, 350 and 500 g/ton nucleoforce®, respectively from zero day till the end of the experiment. At days 21 and 49 of age, two birds from each replicate were randomly chosen, slaughtered, and dissected to collect blood and tissue samples. The concentrations of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in serum were calculated as an indicator for the antioxidant status. Gene expression of insulin-like growth factors 1 (IGF-1) in liver, interleukin-1β (IL-1β) and interleukin-6 (IL-6) in spleen was performed using quantitative real-time PCR (qRT-PCR). The obtained results revealed that the highest nucleoforce® concentration (500g/ton) resulted in time dependent significant increases in both SOD and GPx. This study also indicated that nucleotides supplementation resulted in significant up regulations of growth and immune related gene expression with the best results were obtained with the highest nucleotides concentration. In conclusion, dietary nucleoforce® inclusion can improve the performance of broiler chickens and enhance their antioxidative and immune status.

Bucks reproductive performance can be improved by artificial insemination technique, that affected by many factors the most important one is semen quality, which is affected by the methods of semen preservation cooling and freezing. The goal of this study was to assess the effect of adding different concentrations of antioxidant as Nano-selenium to the diluted buck semen during preservation by cooling in both summer and winter seasons. Eight mature healthy Zaraibi bucks were used in the study. Semen samples were collected by an electro- ejaculator twice per week during summer 2021 (August, September) and winter (January, February). Samples were pooled and diluted by extender then divided to experimental groups: Group 1: Nano-selenium: 100 μL / 100 ml and Group 2: 200 μL / 100 ml.  Then the groups cooled for measuring some semen parameters and seminal antioxidant activities after 1 h from adding and at 8 hours then every 8 hrs up to 64 hr. Nano selenium 200 μL / 100 ml has a favorable impact on diluted buck semen during cooling preservation during summer and winter.