From several stores and butchers in Mansoura city, Dakahlia Governorate, Egypt, 120 samples of sausage, beef burger, minced meat, luncheon, hot dog and canned meat were collected (20 each). The samples examined for detection of total mould count and identification if mould into genera and species in addition to quantification of aflatoxin B1, B2, G1 and G2. Mould detected in 100% of all examined raw meat products meanwhile, detected in 25%, 30% and 15% of examined luncheon, hotdog, and canned meat, respectively. Heat treated meat products significantly (P<0.05) contained lower mould count than raw meat products.  Eight mould genera detected in all examined meat products with varying percentages in descending order Aspergillus > Penicillium > Cladosporium > Sporotricum > Alternaria > Mucor > Fusarium > Curvularia . The mean values of aflatoxin B1 were 0.78±0.21, 1.1±0. 55, 1.54±0.40, 0.052±0.032, 2.21±0.87 and 1.88±0.41 µg/kg in sausage, beef burger, minced meat, luncheon, hot dog and canned meat, respectively. Minced meat significantly lower than other examined meat products in level of aflatoxin B1(P< 0.05). Aflatoxin B2 and G2 not detected in all examined samples. The aflatoxin G1 detected in two samples of beef burger with a mean value 1.15±0.065 µg/kg and in one sample of canned meat 0.62 µg/kg. A food safety management system as hazard analysis and critical control points should be adopted by meat producers in order to protect human health.

One of the most desired and promising diets in Egypt is beef products. It is an enriched media for mycotoxins. The occurrence of aflatoxigenic Aspergillus species is inspected in commercial beef products, HPLC-quantitative amount of aflatoxin B1, B2, G1, and G2 excesses, and genetic identification of aflatoxin regulatory gene (aflR1) by conventional PCR. Two hundred and forty commercial products (minced meat, beef kofta, beef sausage, beef burger, beef luncheon, frozen meat, beef frozen liver, chicken luncheon, chicken burger, chicken frozen liver, mloha, and fesikh; n=20 for each) were collected from different markets at Aswan City, Egypt. Enumeration, isolation, and identification of mold species were carried to each sample. The amount of aflatoxins was measured using HPLC. Genetic identification of the aflR1 gene in Aspergillus was performed using PCR. Mloha samples recorded the highest total mold count whereas the beef luncheon recorded the lowest mould count. Four fungal genera were identified and Aspergillus spp recorded the main with an incidence of 25.8%. By PCR, the aflR1 gene was productively augmented in all the tested Aspergillus spp. The findings illustrated that among the samples that were examined; the prevalence of AFB1 was 65%, followed by AFG2 at 63%, AFB2 at 40%, and AFG1 at 30%. Additionally, mloha (724.2±14.6), poultry frozen liver (288±6.7), and beef frozen liver (91.6±12.2) had higher mean values of total aflatoxins contamination than other samples. Every sample that has been analyzed shows a positive correlation between the amount of reduced total mycotoxins found in the samples and the increased dose of gamma irradiation used to treat the samples. Conclusion: Aflatoxin is frequently linked to meat, poultry, and fish, as well as the products made from these foods. The production of aflatoxin in meat, as well as the products made from it, creates a danger to the public’s health. Thus, the most effective way to prevent aflatoxigenic mould contamination during the product’s production stages is to apply stringent hygienic standards when processing meat products and to use high-quality flavoring agents as spices.

Objective: Aspergillosis is a disease that affects several species of birds and causes substantial losses in the poultry business. The purpose of the investigation was to identify the pathogen responsible for a respiratory outbreak among juvenile ducklings. Materials and Methods: An epidemic of Aspergillosis infected a total of 800 Muscovy ducks that were being reared in El-Beheira Governorate. Tissue samples were obtained to isolate suspected fungi from diseased birds and the hatchery environment. In addition, identification and molecular characterization were performed on the obtained fungal isolates. Results: Affected birds displayed acute respiratory manifestations such as difficulty breathing, gasping for air, nasal discharge, and a mortality rate of up to 28.1%. Postmortem examination revealed bronchitis, tracheitis, congested lungs, air sacculitis, severe multifocal granulomatous pneumonia, a congested, enlarged liver, and a congested kidney with nephritis. Mycological examination revealed seven Aspergillus (A.) spp. isolates from ducklings and six from hatcheries. Isolate colonial morphology and microscopical examination were as follows: A. fumigatus, A. niger, Syncephalastrum racemosum, and four untypable isolates. These isolates were further identified by polymerase chain reaction (PCR), and the internal transcribed spacers (ITSs) gene was detected. Four representative isolates were submitted for sequencing and further phylogenetic analysis. The source of duckling infection might be linked to the hatchery environment due to the observed similarity of isolates from both affected birds and the hatchery, as evidenced by phylogenetic analysis. Conclusion: Our findings demonstrated the significance of appropriate hatchery control in preventing infection in young ducklings. Furthermore, the use of molecular identification techniques would be helpful for tracing the source of infection and rapid diagnosis of Aspergillus in the field. [J Adv Vet Anim Res 2023; 10(4.000): 763-772]