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The ability of in vitro cultured bovine oviduct epityelial cells in binding and maintaining motility of bull sperm
1999
Roh, S.H. (Hankyong National University, Ansung (Korea Republic). Department of Animal Life Resources) | Lee, B.C. | Hwang, W.S. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
The aim of these experiments was to investigate the effects of bovine oviduct epithelial cells(OEC) derived from different segments to bind sperm binding and maintain their motility in vitro. In experiment 1, the number of sperm attached to OEC derived from isthmus or ampulla, the motility of unattached sperm during co-culture and fertilizing ability were assessed. In experiment 2, heparin treated sperm 9hsp) or no treated sperm (nsp) were used to evaluate OEC binding ability ofcapacitated sperm. In experiment 1, regardless ofthier origin, approximately 65% of the sperm were attached to OEC within 2h. From 6h of co-culture, the numbers of unattached sperm on ampullary OEC were significantly higher than those on isthmic OEC (p0.005). From 12h of co-culture, the motility of unattached sperm on isthmic OEC were significantly higher than those on ampullary OEC(p0.05). The cleavage rate of oocytes inseminated on OEC derived from isthmic segment was also significantly higher than those from ampullary segment (p0.01). In experiment 2, the numbers of unattached hsp on OEC were significantly higher than those of controls(p0.01), between 2~24h examination. From 12h of co-culture, the motility of unattached nsp were sighificantly greater than those of hsp(p0.01). These results show that bovine OEC derived from the isthmus play more important role(s) for sperm binding, maintaining motility and fertilization in vitro than those from the ampulla, and heparin induced capacitation may change sperm binding ability on OEC in vitro.
显示更多 [+] 显示较少 [-]Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos 1. Embryo cloning using ovum pick-up (OPU), electric cell fusion and in vitro culture system
1998
Hwang, W.S. | Roh, S.H. | Lee, B.C. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Shin, T.Y. (National Veterinary Research Institute, Anyang (Korea Republic))
Relationship between estradiol-17beta and IGF-I receptor expression in primary cultured rabbit renal proximal tubule cells
1997
Han, H.J. | Nam, S.A. | Park, K.M. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
Comparison of Ag-NORs stain and [3H]thymidine incorporation in antiproliferative effect of caffeine on NIH3T3 cells
1990
Kim, S.H. (Korea Atomic Energy Research Institute, Seoul (Korea Republic). Korea Cancer Center Hospital) | Lee, C.S. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine)
