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Divergent diagnosis from arthroscopic findings and identification of CPII and C2C for detection of cartilage degradation in horses
2010
Lettry, V., Hokkaido Univ., Sapporo (Japan) | Sumie, Y. | Mitsuda, K. | Tagami, M. | Hosoya, K. | Takagi, S. | Okumura, M.
The objective of this study was to investigate the changes in synovial fluid concentration of collagen type II cleavage site (C2C) and pro collagen II C-propeptide (CPII), markers of joint cartilage degeneration and synthesis, respectively, in horses with intraarticular fracture or osteochondrosis dissecans (OCD), and to examine the relationship between arthroscopic findings and these biomarker levels. Synovial fluid was collected from 36 joints in 18 horses (6 fractures and 12 OCDs). Samples from contralateral normal joints, when available, served as controls (n=12). Concentrations of C2C and CPII were measured using enzyme-linked immunosorbant assays. Moreover, the severity of the cartilage degradation was graded arthroscopically in 16 horses, and the correlation between the C2C and CPII levels and the arthroscopic scores were investigated. Compared to the control, the concentration of C2C was increased in OCD joints but not in fracture joints, whereas the concentration of CPII was increased in fracture joints but not in OCD joints. Within each disease group there was no correlation between biomarker levels and arthroscopic findings. Therefore, although C2C and CPII have diagnostic potential further knowledge is required to provide accurate analysis.
显示更多 [+] 显示较少 [-]Strain differences of cerebral ventricles in mice: Can the MRL/MpJ mouse be a model for hydrocephalus?
2009
Hino, K.(Hokkaido Univ., Sapporo (Japan)) | Otsuka, S. | Ichii, O. | Hashimoto, Y. | Kon, Y.
Hydrocephalus is an intractable disease characterized by the excessive accumulation of cerebrospinal fluid (CSF) in the cerebral ventricles. There are many cases in both human and animals; however, the cause and mechanism of its development is not clearly understood. In this study, differences of cerebral ventricles in 5 inbred mice strains (MRL/MpJ, C57BL/6, C3H/He, DBA/2 and BALB/c) were investigated by histological techniques to determine the possibility of a new animal model for hydrocephalus. Our analysis showed that significant differences in the volume and the surface area of lateral ventricles in the 5 inbred strains, with MRL/MpJ mice having the largest lateral, third, aqueduct and fourth ventricles. In addition, when MRL/MpJ mice were compared to BALB/c mice on 0 day after birth, the former already had larger lateral ventricles than the latter. Although there was no significant difference in the ratios of ependymal cell types in MRL/MpJ mice and BALB/c mice, the number and the diameter of lipid droplets in MRL/MpJ mice were, interestingly, smaller than those in BALB/c mice. It is well known that ependymal cells absorb nutritional substances in CSF by endocytosis, suggesting the possibility that their decrease may relate to the larger cerebral ventricles in MRL/MpJ. In conclusion, MRL/ MpJ mice have greater volumes in cerebral ventricles than other strains and may be useful for a model showing high susceptibility to hydrocephalus.
显示更多 [+] 显示较少 [-]A rapid and highly sensitive method for diagnosis of equine influenza by antigen detection using immuno-PCR
2001
Ozaki, H. (Hokkaido Univ., Sapporo (Japan)) | Sugita, S. | Kida, H.
The canine alkaline phosphatases: A review of the isoenzymes in serum, analytical methods and their diagnostic application
1998
Syakalima, M. (Hokkaido Univ., Sapporo (Japan)) | Takiguchi, M. | Yasuda, J. | Hashimoto, A.
This paper reviews the alkaline phosphatases in canine serum, the analytical methods used for qualitative and/or quantitative detection of these isoenzymes, and the diagnostic significancy of each of these isoenzymes. The paper further describes some of the latest advances of our knowledge of the canine alkaline phosphatases and possible areas of future research
显示更多 [+] 显示较少 [-]Diagnostic ultrasound imaging of vegetative valvular endocarditis in cattle
1987
Yamaga, Y. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Too, K.
Enzyme-linked immunosorbent assay (ELISA) as a diagnostic tool for Guatemalan onchocerciasis using a bovine filaria (Onchocerca gutturosa) antigen and blood samples collected on filter paper
1983
Ito, M. | Lujan-T, A. (Servicio Nacional de la Erradicacion de la Malaria, Ministerio de Salud Publica (Guatemala)) | Fukumoto, S. | Kamiya, M.
Development of ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis with truncated 34 kDa proteins
2006
Malamo, M.(Hokkaido Univ., Sapporo (Japan)) | Sakoda, Y. | Ozaki, H. | Kida, H.
To develop ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis), the carboxyl termini of the 34 kDa proteins of M. paratuberculosis and Mycobacterium avium subsp. avium (M. avium) were expressed in Escherichia coli expression system. Antibodies specific to M. paratuberculosis were detected with the truncated 34 kDa protein of M. paratuberculosis in ELISA after pre-absorption of serum samples with the truncated 34 kDa protein of M. avium. All the serum samples from cattle confirmed to be infected with M. paratuberculosis were positive and those from healthy cattle were negative in the present ELISA system. These results indicate that the established ELISA detects antibodies specific to M. paratuberculosis with high specificity and sensitivity and is an useful tool for the screening of Johne's disease.
显示更多 [+] 显示较少 [-]Detection of viral genome in non-neural tissues of cattle experimentally infected with bovine herpesvirus 1
1996
Mweene, A.S. (Hokkaido Univ., Sapporo (Japan)) | Okazaki, K. | Kida, H.
Auscultatory and phonocardiographic studies on the cardiovascular system of the newborn thoroughbred foal
1987
Machida, N. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Yasuda, J. | Too, K.
A perspective on Theileria equi infections in donkeys
2009
Kumar, S.(National Research Centre on Equines, Haryana (India)) | Kumar, R. | Sugimoto, C.
The donkey population has remained unchanged in the last two decades despite a decrease in the overall population of equids, emphasizing the usefulness of the donkey as a draught and pack animal. Piroplasmosis in donkeys, caused by Theileria equi and Babesia caballi, has been recognized as a serious problem of major economic importance as the affected animals manifest decreased working capacity, loss of appetite, etc. In tropical countries, T. equi infections are more wide-spread and pathogenic than those caused by B. caballi. Donkeys usually remain asymptomatic carriers with positive antibody titres throughout life. Transmission of infection occurs from animal to animal through ticks such as Hyalomma spp. Rhipicephalus spp. and Dermacentor spp. The clinical form of the disease is diagnosed by peripheral blood smear examination, but in carrier donkeys it is very difficult to demonstrate the parasite in stained blood smears as the parasitaemia is extremely low. For diagnosis of such low grade infection or carrier animals, serological tests and DNA-based molecular diagnostic techniques, which are discussed in the present review, have become mandatory. Currently, there is no suitable pharmacotherapy available to clear the T. equi infection from affected donkeys, though some new drugs and drug combinations used against this disease condition have been discussed. In the present situation, there is an urgent need for international cooperation and coordination for development of sensitive molecular diagnostic tools and effective pharmacotherapies for curtailment of the disease condition. Hence, it is imperative to develop and exchange reagents and technology developed through human resource sharing in the interest of sustainability of donkey husbandry.
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