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Sensitivity and specificity of Indirect Enzyme Linked Immuno sorbent Assay (ELISA) for diagnosis of hydatidosis in dromedary camels using hydatid cyst fluid antigens.
2020
A. M. El-Kattan1 | Aml M. Abdel-Ra’ouf2 | R. R. Yousef3 | T. R. AbouElnga1 | W.M.A. Mousa4
Examination of 528 (450 males and 78 females) dromedary camels slaughtered at Cairo abattoir revealed that a total of (93) 17.6 % were infected with hydatidosis. Post mortem examination revealed that infection was restricted only in the lungs and the liver of infected camels. Among the 93 hydatidosis infected camels, lungs were the most frequently infected 88 (94.623%) compared with liver 5 (5.376%). ELISA test using partially crude antigen and purified antigen is important for the early diagnosis of cystic echinococcosis as most cases in the early stages of infection are asymptomatic. Sensitivity of ELISA using the crude antigen was 82.758% while sensitivity of the partially purified antigen was 79.310 %. On the other hand specificity of the crude antigen was estimated as 62.5 % and specificity of partially purified antigen as 75.0 %.
显示更多 [+] 显示较少 [-]Study on Toxoplasma gondii, Leptospira spp., Coxiella burnetii, and Echinococcus granulosus infection in veterinarians from Poland
2018
Wójcik-Fatla, Angelina | Sroka, Jacek | Zając, Violetta | Zwoliński, Jacek | Sawczyn-Domańska, Anna | Kloc, Anna | Bilska-Zając, Ewa | Chmura, Robert | Dutkiewicz, Jacek
Introduction: Exposure to zoonotic factors in veterinary practice is closely related to the nature of the work. The main aim of the study was to determine the risk of selected zoonotic infections among the occupational group of veterinarians in Poland. Material and Methods: Blood samples of 373 veterinarians (162 males and 211 females) from 12 provinces of Poland were collected by the venipuncture of a forearm for serological tests. Commercial immunoenzymatic tests (ELISA) were used for detection of specific IgG antibodies to Echinococcus granulosus, IgM and IgG to Leptospira spp., and IgM, IgA, and I and II phase IgG to Coxiella burnetii. Enzyme-linked fluorescence assays (ELFA) were used to detect IgM and IgG antibodies to Toxoplasma gondii. Results: Positive results were found in 209 (56.0%) veterinarians for at least one of the examined diseases. The overall proportion of participants found to have specific Toxoplasma gondii antibodies in the IgM and/or IgG assays amounted to 44.5%. The presence of Coxiella burnetii antibodies was found in 16 (4.3%) subjects, while Leptospira spp. antibodies were detected in 63 (16.9%) veterinarians. Among the 373 veterinarians examined, no Echinococcus granulosus antibodies were found. Conclusion: Results of the study seem to indicate a slightly elevated risk of Toxoplasma gondii infection and a moderate risk of infection with Leptospira spp. and Coxiella burnetii in veterinarians.
显示更多 [+] 显示较少 [-]Hydatid hepatic-broncho-pleural (hepatopulmonary) fistula caused by Echinococcosis granulosa: a zoonotic case report
2018
Tarik Ibrahem Ali | Omar Emad Ibrahim | Imad I. Al-Sultan
A rare case is presented of a 58-year-old woman who developed ahepatic-bronchopleural fistula following a hydatid hepatic cyst complication. The hepatic-bronchopleural fistula was diagnosed when patient complained of severe repetitive attacks of productive cough of brown yellow to dark colour sputum biliptysis (bile in cough) with vomiting associated with night sweating and fever, for the past one month. She also complained of right side chest and abdominal pain for the past 3 years. This paper describes a unique case of hepatic-bronchopleural fistula caused by hydatid disease, emphasising the clinical feature and its surgical management. Hydatid disease is a zoonotic infection dueto the damage caused by Echinococcus granulosus. E. granulosus, also called the hydatid worm, hyper tape-worm or dog tapeworm, is a cyclophyllid cestode that parasitises the small intestine of canids as an adult, but which has important intermediate hosts such as livestock and humans, where it causes cystic echinococcosis, also known as hydatid disease. It is hoped thatthis case report will add to the meagre case management reports of hepaticbronchopleural fistula in literature.
显示更多 [+] 显示较少 [-]Prevalence of bovine cysticercosis and hydatidosis in Jimma municipal abattoir, South West Ethiopia
2009
Tolosa, T.(Jimma University College of Agriculture and Veterinary Medicine School of Veterinary Medicine) | Tigre, W.(Jimma University College of Agriculture and Veterinary Medicine School of Veterinary Medicine) | Teka, G.(Jimma University College of Agriculture and Veterinary Medicine School of Veterinary Medicine) | Dorny, P.(Ghent University Faculty of Veterinary Medicine Laboratory of Parasitology,Institute of Tropical Medicine Department of Animal Health)
A cross-sectional study to determine the prevalence of bovine cysticercosis and hydatidosis was conducted from October 2007 to March 2008 in cattle slaughtered at the Jimma municipal abattoir. Cyst distribution and viability of bovine cysticercosis and hydatidosis were also determined. A total of 512 carcasses were inspected of which 15 (2.93%) and 161 (31.44%) were infected with Taenia saginata metacestodes and hydatid cysts, respectively. From a total of 109 cysticerci collected from infected carcasses, 47 (43.12 %) were viable. The anatomical distribution of the cysticerci was, shoulder muscle (39.5°%), heart (33.9 %), neck muscle (13.8°%), tongue (10.1°%), masseter muscles (1.8°%) and diaphragm (0.9°%). Of the 1171 hydatid cysts collected 223 (19.0°%) were fertile, 505 (43.1 °%) sterile, 49 (29.8 °%) calcified and 94 (8.0 °%) contained pus. A greater proportion of fertile cysts were found in the lungs than in other organs. It was concluded that these zoonotic cestodes deserve due attention to safeguard public health, and that further studies are needed on genotyping, epidemiology and public health importance of Echinococcus granulosus in the study area.
显示更多 [+] 显示较少 [-]Echinococcosis in Kenya: Transmission characteristics, incidence and control measures
1990
Gathura, P.B. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Kamiya, M.