A total of seventy four egg samples were collected from different markets sources, to investigated for Salmonellaspp. the bacteria was isolated and identified using culturing on selective media, in addition to, biochemical and serotyping by monovalent antisera. Multiplex Polymerase Chain Reaction(PCR) detection of theinvA; and DH genes was used for conformation of the identification of the Salmonella spp.. Twenty eight samples from seventy four eggs provide positive results with PCR as Salmonella spp. depending on InvA gene which is the target for the identification at the genus level.

In the study, 348 bacterial isolates comprising of Escherichia coli (100), Klebsiella aerogens (45), Proteus mirablis (35), Peudomonas aerogens (20), Staphylococcus sp. (78), Streptococcus sp. (13), Bacillus sp. (57) were isolated from egg shell and egg contents of 150 egg samples collected from local market in Patna (Bihar). The isolates were subjected to antibiogram. This study demonstrated that egg from organized farm microbial contamination than unorganized poultry farm reflecting the effect of environment, storage and transportation on microbial quality of eggs.

Objectives: This study was designed to assess the contamination of enterovirulent Escherichia coli with table eggs at Mansoura, Egypt. Materials and methods: A total of 100 commercially available table eggs were randomly collected from various groceries and supermarkets at Mansoura, Egypt. The samples were screened for the presence of E. coli through conventional bacteriological and biochemical analyses followed by confirmation by polymerase chain reaction. Results: Overall, 18% (n=18/100) samples were found to be contaminated with one or more E. coli isolates. All possible E. coli colonies (n=52) appeared on MacConkey agar plates during the screening process were picked for further analysis. Among the 52 suspected isolates, 24 were confirmed as E. coli, which were further serotyped using polyvalent E. coli antisera. In this study, 9 different E. coli serotypes namely O78, O114, O2, O44, O1, O125, O128, O124 and O26 were identified. Out of these 9 serological strains, 5 (O78, O2, O44, O125, O124 and O26) were positive for eae gene, and 3 (O44, O1 and O128) were positive for stx2 gene. Two serological strains (O44 and O1) were positive for both stx1 and eae genes, while O125 and O114 were positive for stx2 and eae genes. Two strains (O78 and O128) were found to be positive for all three genes (stx1, stx2 and eae). Conclusion: Ensuring hygienic measures can effectively reduce the microbial load from table eggs. [J Adv Vet Anim Res 2016; 3(1.000): 1-7]

Into the programme hen selection of Belarus Autoseksnyj cross there was introduced the indeks of the so-called quality of eggs which is defined by removal defective on quality of a shell of eggs in 30 and 52-week age. The size of variability of an indicator of 16,0-25,4% and hehtability 0,3-0,4 allows to lead selection on increase in an exit of qualitative eggs. Influence on quality of eggs with defects of a shell of age of hens, intensity eggs, weights of eggs, a linear accessory is established

Scientific experiments on determination of the optimal doses kizelgur for import substitution of shells in poultry diets was realized in the conditions of the Republic of Belarusby an example of 4 groups of laying hens of Khajseks korichnevyj cross. Hens of the first (controlled) group were given full ration applied at the tested poultry farm which included 5% of shells. Hens of the other three experimental groups were given complete feedstuffs with inclusion of mineral feed kizelgur: hens of the second group – in dose 2% out of dry matter mass; third group - 3%; for the fourth group - 4%. The research results showed that application of mineral feed additive kizelgur increased hen productivity in experimental groups on 2,8%; 5,9%; 7,2%, respectively. In the experimental groups there was an increasing of egg yields on 2,8%; 5,9%; 7,2%, respectively. The best indexes of poultry livability were stated in the third group (on 5,5% higher than in control). The similar tendency was stated in course of analysis of egg weight, shell thickness and calcium content of shell. Kizelgur application led to increasing of egg mass in tested groups: on 0,46% (2 group), 1,6% (3 group) and 2,4 % (4 group). At the age of 340 days shell thickness in experimental groups was on higher 3,3% (2 group), 0,7% (3 group) and 8,7 % (4 group) than in control. The analyzed additive increased the natural resistance of hens that was proved by indexes of bacterial growth-inhibitory activity of blood serum and lysozyme activity. There noted tendency of total protein increasing. Studying of immunological indexes showed that they were the most evident in the fourth experimental group