The study was conducted to determine the role of Ca2+ and K+ channels in Moringa oleifera flowers extract-induced myometrial contractility of buffalo uterus. Myometrial strips were prepared, mounted in an organ bath containing Ringer Locke solution maintained at 37oC and responses recorded using a kymograph. Moringa oleifera flowers extract (MOFE) produced quite conspicuous and concentration-dependent contractile effect on buffalo myometrium and the EC50 value was found to be 31.62 ìg/ml. Verapamil (10-12M) not only completely inhibited normal spontaneous rhythmic contractions of myometrial uterine strips but also inhibited MOFE-induced spontaneous rhythmic contractions. Ethylene glycol tetra acetic acid (EGTA; 0.1-0.2mM) reduced both the frequency and amplitude of spontaneous contractions and also produced around 28% reduction in the amplitude of MOFE-induced contractile effect. Similalrly, pinacidil (10-6M-10-5M), glibenclamide (10-5M) and 4- amino pyridine (4-AP; 1mM) pretreatments inhibited spontaneous contractions as well as the contractile effects of MOFE (250ìg/ml). Based on the results of present study, it may be inferred that both the extra- and intracellular Ca2+ are very vital for the oxytocic effect of MOFE. Functional presence of K+ ATP, K+ V and/or K+ Ca channels in buffalo myometrium and their involvement in mediating MOFEinduced contractility can not be ruled out. Moringa oleifera flowers possessed promising oxytocic activity and can be exploited in drug-development programme for evolving natural and effective oxytocic or abortifacient.