Identification of processed animal protein in animal feedstuffs wasperformed under the feed safety programme to ensure that the products used locally to feed the livestock are safe and properly labeled to prevent unnecessary incidence that will affect both animal and human.A “silica-membrane technology” method was applied based on its fast and effective purification of nucleic acids from various matrices. The silica membranes were optimized for high DNA recovery and lowbinding efficiency for impurities. Results from 58 with various kinds of samples showed negative of unwanted processed animal protein.

This study was conducted to investigate whether dietary enzymes alter the caecal microbial profile of broilers fed sorghum-based diets. Four sorghum-based diets (918 g sorghum/kg diet) were prepared. One was the control diet and three had enzymes (xylanase, phytase andprotease) added. Broilers, 35-day-old, were reared (8 birds/cage) in an environmentally controlled shed and randomly allocated to replicated (n=4) assay diets and free access to feed and water all time. On day-42, birds were euthanized and caecal contents collected, pooled on a per/pen basis and frozen (-20 °C). The DNA was extracted from caecal samples using a bead-beating protocol and the V2V3 regionof the bacterial 16S rRNA gene amplified by PCR. Amplicons were separated on sequence difference using Denaturing Gradient Gel Electrophoresis (DGGE) and microbial profiles generated and compared.The DGGE profiles, when analysed, indicated that there was approximately 80% similarity between caecal microflora in all types of the diet treatments. This suggests that there was no overalldifference between any of the profiles and therefore the addition of different types of feed enzymes in a sorghum-based diet had no impact on the overall composition of the broiler caecal microflora.

Forty-nine fresh goat’s milk samples produced by local farmers and sold in market for public consumption as well as raw goat milk in Johor, Malaysia were analysed for total plate count(TPC) , E. coli, Coliform, Brucella melitensis, Brucella abortus,Coxiella burnetii as well as aflatoxin M1 (AFM1) content, as measures for food safety. The mean counts per ml for TPC were 4.90 x 105, 6.50 x 105, 1.60 x 105 and 1.48 x 106 for pasteurised, unpasteurised and unknown (status of pasteurisation) milk sold in the market as well as the raw milk from milkcollection center (MCC), respectively. Among pasteurised samples, only one had TPC count higher than the permitted level whereas the rest were all within the permitted level. The mean counts per ml for E. coli were <1.00 x 102 for pasteurised and unknown milkwhereas 1.67 x 101 for unpasteurised and 1.18 x 102 for raw milk. The mean counts per ml for coliform were 9.53 x 103, 9.76 x103, 1.20 x 102 and 1.16 x 104 for pasteurised, unpasteurised, unknown milk and raw milk, respectively. Overall, no significantdifferences on the bacterial counts in both pasteurised and unpasteurised milk. All milk samples were negative of B. melitensis and B. abortus, but one unknown sample fromthe market and two raw samples from MCC were positive of C. burnetii through the ELISA test. The unknown sample from the market showed the presence of C. burnetii when further analysed microscopically. Meanwhile, no sample exceeded the permitted level of AFM1 in milk.

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent for the coronavirus disease 2019 (COVID-19) pandemic. COVID-19 is contagious and fatal to humans. In the context of the COVID-19 pandemic, significant concerns on food safety and security are rising due to potential interspecies transmission. As such, surveillance of SARS-CoV-2 on imported meat and animal parts is carried out and reported in this study to safeguard food safety and security. Overall, none of the 225 samples from various livestock (buffaloes, cattle, goat and pig) imported from seven countries were tested positive for SARS-CoV-2 with quantitative reverse transcription polymerase chain reaction (RT-qPCR) from July 2020 to November 2021. This study finding serves as a baseline data for SARS-CoV-2 in imported meat and animal parts. Notably, this study accentuated the importance of active surveillance to prevent zoonosis and to safeguard food safety and security.

The poultry industry is the most advanced and independent among livestock industry in Malaysia with over 100 percent self-sufficient level (SSL) since 2004. The Ministry of Agriculture and Food Industry (MAFI) has launched National Agrofood Policy 2021-2030 (DAN 2.0) in October 2021. One of the objectives is to increase the poultry’s SSL to 140.2 % by 2030, an increase from 104.10 % in 2020. The objective of this study was to analyze the production cost of selected commercial broiler farms in Malaysia. The scope of the study was to evaluate the economic assessment of broiler production in order to understand the industry widely and also to identify which segments along the production line need to be improved and at the same time to ensure that this industry remains competitive and productive. Production cost data were obtained from selected commercial broilers via questionnaire forms. The result showed that the average broiler production cost is RM4.40/kg. Small farms have higher total average cost per kg, which is RM4.70, followed by commercial scale farms at RM4.43 and medium-scale farms at RM4.29, respectively. Feed cost contributes 66 % of the total production cost of whole expenditure followed by day-old chick’s cost, 19 %. The average profit estimation is RM0.65/kg based on RM5.05/kg for the ex-farm price of live poultry.