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Herd evaluation of Fasciola hepatica infection levels in Louisiana cattle by an enzyme-linked immunosorbent assay.
1987
Welch R.D. | Smith P.H. | Malone J.B. | Holmes R.A. | Geaghan J.P.
Pharmacokinetics of diacetoxyscirpenol in cattle and swine: effects of halothane.
1987
Coppock R.W. | Swanson S.P. | Gelberg H.B. | Koritz G.D. | Buck W.B. | Hoffmann W.E.
DNA homology studies of leptospires of serogroups Sejroe and Pomona from cattle and swine.
1986
Le Febvre R.B. | Thiermann A.B.
Scanning electron microscopy of bovine coreneas irradiated with sun lamps and challenge exposed with Moraxella bovis.
1986
Vogelweid C.M. | Miller R.B. | Berg J.N. | Kinden D.A.
Characterization of Tritrichomonas foetus antigens, using bovine antiserum.
1986
Hall M.R. | Huang J.C. | Ota R. | Redelman D. | Hanks D. | Taylor R.E.L.
Vaccination of cattle against ringworm caused by Trichophyton verrucosum.
1986
Gudding R. | Naess B.
Brachygnathia in a herd of Angus cattle.
1985
Heidari M. | Vogt D.W. | Nelson S.L.
Modified indirect fluorescent antibody test for the serodiagnosis of Anaplasma marginale infections in cattle.
1985
Montenegro James S. | James M.A. | Ristic M.
Influence of time after the removal of nocodazole from nuclear donors on the development of reconstituted embryos in bovine nuclear transplantation.
1995
Tanaka H. | Takahashi Y. | Hishinuma M. | Kanagawa H.
The present study examined the influence of post-cleavage time of nuclear donors on the development of reconstituted embryos in bovine nuclear transfer. Blastomeres of 16-cell stage embryos derived from in vitro-maturation, fertilization and culture were used as nuclear donor source. They were treated with 10 mu-M nocodazole for 12 hr. Blastomeres that cleaved within 3 hr after the removal of nocodazole were used-for the study. Metaphase II (M-II) oocytes were used as recipient cytoplasm. IN experiment 1, donor blastomeres at 6, 11 and 15 hr after the removal of nocodazole and donor blastomeres no treated with nocodazole were transferred into ethanol-exposed and enucleated oocytes. The reconstituted embryos produced by donor blastomeres oat 6 hr after the removal of nocodazole had a significantly higher developmental rate to the blastocyst stage than those at 15 hr and the untreated groups (P<0.01). In experiment 2, blastomeres at 6 hr after the removal of nocodazole used as nuclear donors were transferred into ethanol-exposed and enucleated M-II oocytes. The reconstituted embryos with ethanol-exposed and enucleated oocytes as recipient cytoplasm had a significantly higher rate of initial-cleavage (P<0.05) and development to the blastocyst stage (P<0.01) than non ethanol-exposed and enucleated M-II oocytes. These results demonstrate that the development of reconstituted embryos was improved when cleaved donor blastomeres after the removal of nocodazole were immediately transferred (at 3-6 hr post-cleavage) into activated enucleated oocytes by exposure to ethanol.
显示更多 [+] 显示较少 [-]Effect of PHA and conditioned medium on blastogenesis and rosette formation of bovine circulating blood lymphocytes.
1994
Kang S.W. | Yoon C.Y. | Song H.J.
