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Identification of Hanwoo and Holstein meat using MGB probe based real-time PCR associated with single nucleotide polymorphism (SNP) in Melanocortin 1 receptor (MC1R) gene
2005
Park, S.D. (Chonnam National University, Gwangju, Republic of Korea) | Kim, T.J. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.I. (Chonnam National University, Gwangju, Republic of Korea), E-mail: jaeil@chonnam.ac.kr
The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo meat, we performed a single nucleotide polymorphism (SNP) analysis in Melanocortin 1 receptor (MC1R) gene using TaqMan∨® MGB probe-based real-time PCR. Two specific probes (one for Hanwoo and the other for Holstein and Black angus) were designed.
显示更多 [+] 显示较少 [-]Sequence analysis and expression of groE gene encoding heat shock proteins of Brucella abortus isolates
2005
Kim, T.Y. (ChoongAng Vaccine Laboratory, Daejeon, Republic of Korea) | Kim, J.Y. (Chungnam National University, Daejeon, Republic of Korea) | Chang, K.S. (University of Kentucky, Lexington, KY, USA) | Han, H.R. (Seoul National University, Seoul, Republic of Korea) | Kim, M.C. (Chungnam National University, Daejeon, Republic of Korea) | Park, C.S. (Chungnam National University, Daejeon, Republic of Korea) | Jun, M.H. (Chungnam National University, Daejeon, Republic of Korea), E-mail: mhjun@cnu.ac.kr
GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains.
显示更多 [+] 显示较少 [-]Rapid differentiation of Hanwoo and Holstein meat using multiplex allele specific polymerase chain reaction protocols
2005
Koh, B.R.D. (Gwangju Metropolitan Health and Environment Research Institute, Gwangju, Republic of Korea), E-mail: barada@nvrqs.go.kr
Here I describe a multiplex allele specific PCR-based approach for the rapid detection between Hanwoo and Holstein meat associated with Melanocortin 1 receptor (MC1R) gene. Specific and universal oligonucleotide primers were used in combination to detect the presence of a single nucleotide polymorphism within the bovine MC1R DNA sequence. The presence of the bovine MC1R gene is indicated by the production of a single control PCR product, whilst positive samples generate an alternative smaller specific product over the same region. The mutations in MC1R104 codon revealed depending on the presence or absence of an indicative fragment amplified from the wild-type allele of this codon.
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