Escherichia coli gene mutations (plasmids, integrons, and transposons) have instigated multidrug resistance (MDR) against various antimicrobials. This study detected antibiotic-resistance genes (genotypic and phenotypic) in E. coli and performed Whole-genome sequencing to discover MDR-associated E. coli genes in mullet fish. E. coli isolates were serologically identified and their antimicrobial sensitivity was tested. E. coli presence was confirmed in 35% of the mullet fish samples. Cephalosporins, tetracyclines, and sulfonamides presented higher efficiency against E. coli. The serological investigation revealed the presence of STEC (O157:H7, 50%), ETEC (O142, 20%), EHEC (O26:H11,15%), and  EPEC (15%) in mullet meat samples. A symmetrical band represented the Subunit B of the Shiga-like toxin (SLT) genewhereas the Heat-labile toxin (LT) gene was found in plasmid and genomic DNA-detected strains. The results revealed the hazardous nature of STEC for mullet meat consumers. The study recommends improving the hygienic conditions during the mullet handling and processing steps, which will minimize  antibiotic usage and resistance.

Diarrhoea in calves is considering a serious clinical problem inBangladesh. Calf is the back bone of the dairy industry. Diarrhoea in farm animals, especially in neonatal calves is one of the most challenging clinical syndromes encountered by large animal Veterinarypractitioners. The present investigation was conducted with the aimed to evaluate the efficacy of ciprofloxacin and gentamycin in the treatment of diarrhoea in neonatal calves. The studied was carried out for a period of six month from January 2013 to June 2013 in Kanaighat, Sylhet, Bangladesh. Calves (n=60) under 10 days of age with clinical signs of diarrhoea were randomly divided into two groups. One group was treated with intramuscular ciprofloxacin at a dose of 5 mg/kg once daily, while other group was treated withintramuscular (IM) gentamycin (dose of 5 mg/kg) once daily for three consecutive days. Calves evaluated clinically from the treatment and the recovery rates for the ciprofloxacin and gentamycin groups afterthree days of treatment were 73.33% and 66.67%, respectively. This trial revealed ciprofloxacine administered through IM route from the first day of the clinical sign observed was more efficacious in calfdiarrhoea compared to gentamycin.