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Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1
2005
Chang, K.S.(Hokkaido Univ., Sapporo (Japan)) | Ohashi, K. | Lee, S.I. | Takagi, M. | Onuma, M.
The meq gene was thought to be only detected in Marek's disease virus serotype 1 (MDV1) including a very virulent strain, Md5, while L-meq, in which a 180-bp sequence is inserted into the meq open reading frame, is found in other strains of MDV1, such as CVI988/R6. However, both meq and L-meq were previously detected by PCR in chickens infected with MDV1, suggesting hat MDV1 may consists of at least two subpopulations, one with meq, the other with L-meq. To further analyze these subpopulations, we analyzed the time course changes in distribution of these subpopulations among T cell subsets from chickens infected with MDV1. Both meq and L-meq were detected in CD4(+) and CD8(+) T cells infected with strain Md5 or CVI988/R6. The shift in MDV subpopulations from one displaying meq to the other displaying L-meq and/or the conversion from meq to L-meq occurred mainly in the CD8(+) T cell subset from Md5- infected chickens. PCR products corresponding to L-meq rather than meq were frequently amplified from the CD8(+) T cell subset from CVI988/R6-infected chickens. These results suggest that a dominant subpopulation of MDV1 changes depending on the T cell subsets, and that L-meq is dominantly present in the CD8(+) T cells which play a role in the clearance of pathogenic agents.
显示更多 [+] 显示较少 [-]Adrenocortical responses to tumor necrosis factor-alpha and interferon-gamma in cattle
2004
Soliman, M. (Hokkaido Univ., Sapporo (Japan)) | Okamoto, S. | Kitamura, H. | Kushibiki, S. | Kimura, K. | Saito, M.
The responses of plasma cortisol and adrenocorticotropic hormone (ACTH) were examined to intravenous injection of recombinant bovine tumor necrosis factor-alpha (TNF-alpha) and interferon-y (INF-gama) in Holstein cows. INF-gamain-duced dose-dependent rises in the plasma levels of both cortisol and ACTH, while TNF-alpha induced comparable plasma cortisol responses with much smaller rises in plasma ACTH. The results suggest a direct stimulatory action of TNF-alpha on cortisol secretion from the adrenal gland in cattle.
显示更多 [+] 显示较少 [-]Immunohistochemical studies on the localization of antigen-bearing phagocytes and lymphocytes in mouse lymph nodes after the germinal center formation
1984
Nakamura, K. | Hashimoto, Y. | Kitagawa, H. | Kudo, N. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
