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Incidence of micronuclei in lymphocytes of pig in the high background radiation area (Cheongwon-gun and Boeun-gun)
2005
Lee, H.J. (Chonnam National University, Gwangju, Republic of Korea) | Kang, C.M. (Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea) | Kim, I.H. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, T.H. (Kyungpook National University, Daegu, Republic of Korea) | Ryu, S.Y. (Chungnam National University, Daejeon, Republic of Korea) | Jo, S.K. (Korea Atomic Energy Research Institute, Daejeon, Republic of Korea) | Kim, S.R. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.H. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.S. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.C. (Chonnam National University, Gwangju, Republic of Korea) | Kim, S.H. (Chonnam National University, Gwangju, Republic of Korea), E-mail: shokim@chonnam.ac.kr | Choi, S.Y. (Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea)
Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy.
显示更多 [+] 显示较少 [-]Peripheral lymphocyte counts in Holstein-Friesian cattle infected with bovine leukemia virus in Korea
2005
Suh, G.H. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea), E-mail: ghsuh@rda.go.kr | Lee, C.G. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.C. (Seojeong College, Yangju, Republic of Korea) | Hur, T.Y. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Kang, S.J. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Son, D.S. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Ahn, B.S. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Kim, N.C. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Lee, C.Y. (Chonnam National University, Gwangju, Republic of Korea)
Hematologic investigations were made on the blood samples taken from bovine leukemia virus (BLV)-seropositive Holstein-Friesian cattle in Korea, and their absolute lymphocyte count was compared with that of BLV-seronegative cattle. The incidence of persistent lymphocytosis (PL) was also determined. The normal bovine lymphocyte count was established on the basis of studies of 656 blood samples taken three times from 297 seronegative animals aged from 0~6 months to over 5 years at 5~6-month intervals. The data were examined according to 7 age groups of samples placed into their respective age groups.
显示更多 [+] 显示较少 [-]Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1
2005
Chang, K.S.(Hokkaido Univ., Sapporo (Japan)) | Ohashi, K. | Lee, S.I. | Takagi, M. | Onuma, M.
The meq gene was thought to be only detected in Marek's disease virus serotype 1 (MDV1) including a very virulent strain, Md5, while L-meq, in which a 180-bp sequence is inserted into the meq open reading frame, is found in other strains of MDV1, such as CVI988/R6. However, both meq and L-meq were previously detected by PCR in chickens infected with MDV1, suggesting hat MDV1 may consists of at least two subpopulations, one with meq, the other with L-meq. To further analyze these subpopulations, we analyzed the time course changes in distribution of these subpopulations among T cell subsets from chickens infected with MDV1. Both meq and L-meq were detected in CD4(+) and CD8(+) T cells infected with strain Md5 or CVI988/R6. The shift in MDV subpopulations from one displaying meq to the other displaying L-meq and/or the conversion from meq to L-meq occurred mainly in the CD8(+) T cell subset from Md5- infected chickens. PCR products corresponding to L-meq rather than meq were frequently amplified from the CD8(+) T cell subset from CVI988/R6-infected chickens. These results suggest that a dominant subpopulation of MDV1 changes depending on the T cell subsets, and that L-meq is dominantly present in the CD8(+) T cells which play a role in the clearance of pathogenic agents.
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