Background: Linguatula serrata is a zoonotic parasite causing Halazoun syndrome in humans. Consumption of raw or semi-cooked infected edible offal induces the infection in human. Objectives: The main objective of this study was to investigate the outbreak and molecular characterization of Linguatula serrata in sheep and goat of Yazd slaughterhouse. Methods: To determine the prevalence and severity of Linguatula serrata, mesenteric lymph nodes of 200 slaughtered sheep and 200 slaughtered goats in the Yazd industrial slaughterhouse were examined. DNA extraction was performed using commercially DNA extraction kit as manufacturers’ protocol. In order to genetic evaluation, the partially 18srRNA gene as a target was amplified using the specific primer pair which was designed by Primer3 software.The PCR product sent for sequencing and the sequence was BLAST. Data were then analyzed using SPSS version 16.0 and by the Pearson correlation test and χ2 at a significance level of 0.01.Results: In the present study, prevalence of the infection of slaughtered goats and sheep was 25.5% and 22.5%, respectively. No statistically significant difference was observed between the prevalence of this parasite in different ages and sexes groups (goats and sheep). The results of genetic evaluation showed no variation between this isolate in comparison with the ones in GenBank. Conclusions: This study was the first report of molecular identification of Linguatula serrate in Iran. Considering high prevalence of infection in domestic animal and lack of knowledge and hygienic practice of the people about consumption of animal offal infection of the people to Linguatula serrata is probable. Therefore, in this context, using appropriate and reliable diagnostic methods for detection of infection in abattoirs as well as educating people on the proper use of animal offal is effective steps to prevent this disease.

In this study a total number of 22 organ samples (including trachea, lung and kidney) from 22 broiler farms from northern Upper Egypt were collected from Mars 2017 to June 2018 from chickens showing clear clinical and pathological signs of Infectious Bronchitis. The samples were prepared and examined by real time RT-PCR for diagnosis of IBV. A total number of 11 samples were positive (50%) which were used for further isolation on SPF eggs by three blind serial passages. Positive samples that showed the pathogenic lesions of IB (curling and dwarfing of embryos) were collected and tested with real time RT-PCR (rRT-PCR) for more confirmation then a part from S1 gene sequence was amplified by RT-PCR and the product was sequenced and the data have been compared with other related IBV strains. The results indicate that the Egyptian virus in this study has an identity percent reached up to 89% with other recent Egyptian isolates. However, it reached 67% with classical vaccine strains like H120 and variant I like CR88 strain. The lowest identity was observed with M41 strain (59%) in this study. The phylogenetic tree compared to other isolates from Middle East and worldwide showed that this isolate is related to the IBV variant 2 group closely related to IBVEg/1265B/2012 strain and the Israeli strain IS/1494/06.

Little is known about latent infection and molecular characterisation of Neospora caninum in sheep (Ovis aries). In this study, 330 sheep samples (180 hearts and 150 brains) were analysed for N. caninum DNA by nested polymerase chain reaction (PCR) targeting the Nc-5 gene. Neospora caninum DNA was detected in 3.9% (13/330) of sheep samples. The parasite’s DNA was detected in 6.7% of heart samples (12/180) and 0.7% (1/150) of brain samples. No clinical signs were recorded from infected or uninfected animals. Sequencing of the genomic DNA revealed 96% – 99% similarity with each other and 95.15% – 100% similarity with N. caninum sequences deposited in GenBank. To our knowledge, this is the first report on the use of PCR to identify latent neosporosis in sheep in Iran. The results of this study have the potential to contribute to our understanding of the role of N. caninum-infected sheep in the epidemiology of neosporosis.

Indigenous duck breed of Assam are popular with considerable production potential with minimal input and mostly reared under semi intensive system of management. These ducks are maintained in all agro climatic zones of Assam and different from other indigenous duck genetic resources available in the country. But the genetic structure of these duck varieties was not fully studied; hence the genetic characterization of Assam ducks was assessed with 23 FAO recommended duck specific microsatellite markers using advanced automated genotyping technique. The analysis revealed that totally 91alleles were observed with the number ranging from 1 (CAUD025) to 7 (CAUD004 and APH009) and an overall mean of 3.957 ± 0.32 across the loci. The mean observed and expected heterozygosities were 0.4444 and 0.5113. All the microsatellite loci were found to be highly polymorphic except CAUD025. In Assam ducks, PIC value ranged from 0.14 (APH001) to 0.71 (CAUD004) with a mean value of 0. 4813. Nearly 14 out of 23 loci had PIC values of more than 0.5 indicating that these markers can be effectively used for genetic diversity analysis. The Chi-square test revealed that among the 23 microsatellite studied, only 12 were in Hardy-Weinberg equilibrium proportions and the rest departed from equilibrium. Selection and non-random mating could be the main reasons for this disequilibrium. The markers used in the study were found to be highly informative, explores high genetic variation in the population which could be exploited for their improvement.