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High resolution protein electrophoresis of equine cerebrospinal fluid.
1997
Furr M. | Chickering W.R. | Robertson J.
Electroencephalogram and evoked potentials in naturally occurring scrapie in sheep.
1986
Strain G.M. | Olcott B.M. | Braun W.F. Jr.
Total protein, albumin quota, and electrophoretic patterns in cerebrospinal fluid of dogs with central nervous system disorders.
1987
Sorjonen D.C.
Canine storage disease characterized by hereditary progressive neurogenic muscular atrophy: breeding experiments and clinical manifestation.
1986
Inada S. | Yamauchi C. | Igata A. | Osame M. | Izumo S.
Culture of glial cells isolated from the spinal cord of demyelinating mice infected with Theiler's virus: An immunocytochemical study.
1991
Shin T.K.
Effect of electromagnetic field exposure on mouse brain morphological and histopathological profiling 全文
2020
Zymantiene, Judita | Juozaitiene, Vida | Zelvyte, Rasa | Oberauskas, Vaidas | Spancerniene, Ugne | Sederevicius, Antanas | Aniuliene, Albina
Effect of electromagnetic field exposure on mouse brain morphological and histopathological profiling 全文
2020
Zymantiene, Judita | Juozaitiene, Vida | Zelvyte, Rasa | Oberauskas, Vaidas | Spancerniene, Ugne | Sederevicius, Antanas | Aniuliene, Albina
Mobile phones (MP) and other electronic and communication devices that are used daily expose users to electromagnetic fields (EMF) and contribute to an increasing incidence of neurological disorders. Brain tissue is the closest organ to the MP as it operates, thus the influence of MP radiation on brain tissue is of particular concern, although research is still inconclusive. The present study investigated the possible effect of an EMF (1,350–1,375 megahertz (MHz)) from an MP on morphological and histopathological profiles in the mouse brain. Healthy BALB/c mice were assigned to three equal groups (a control and two experimental groups, n = 10 each). Experimental mice were exposed to EMFs continuously for 72 h, those of experimental group I to a 1,350 MHz field at a specific absorption rate (SAR) of 4.0 W/kg, and group II to a 1,375 MHz field EMF at an SAR of 4.0 W/kg. Brain segmentation and histopathological analysis were applied to detect changes in the morphometric parameters of the brain lobes and identify pathological lesions, respectively. Histopathology results revealed shrinkage of pyramidal neurons, presence of mild perivascular and perineural oedema, and some vacuolation of neurons and glial cells derived from mouse great hemispheres. The lesions also included reduction of Purkinje cells, vacuolisation of neurons and glial cells, and interstitial oedema in the cerebellum. MP distance of 3 cm from the cage may induce appreciable morphological changes in mouse brain structures; therefore, more comprehensive research is essential for assessment of safe distance. These pronounced effects may interfere with the results of laboratory tests on murine experimental models in veterinary or biomedical research.
显示更多 [+] 显示较少 [-]Effect of electromagnetic field exposure on mouse brain morphological and histopathological profiling 全文
2020
Zymantiene Judita | Juozaitiene Vida | Zelvyte Rasa | Oberauskas Vaidas | Spancerniene Ugne | Sederevicius Antanas | Aniuliene Albina
Mobile phones (MP) and other electronic and communication devices that are used daily expose users to electromagnetic fields (EMF) and contribute to an increasing incidence of neurological disorders. Brain tissue is the closest organ to the MP as it operates, thus the influence of MP radiation on brain tissue is of particular concern, although research is still inconclusive. The present study investigated the possible effect of an EMF (1,350–1,375 megahertz (MHz)) from an MP on morphological and histopathological profiles in the mouse brain.
显示更多 [+] 显示较少 [-]Biomarkers of injury to neural tissue in veterinary medicine 全文
2016
Biomarkers of injury to neural tissue in veterinary medicine 全文
2016
There are numerous biomarkers of central and peripheral nervous system damage described in human and veterinary medicine. Many of these are already used as tools in the diagnosis of human neurological disorders, and many are investigated in regard to their use in small and large animal veterinary medicine. The following review presents the current knowledge about the application of cell-type (glial fibrillary acidic protein, neurofilament subunit NF-H, myelin basic protein) and central nervous system specific proteins (S100B, neuron specific enolase, tau protein, alpha II spectrin, ubiquitin carboxy-terminal hydrolase L1, creatine kinase BB) present in the cerebrospinal fluid and/or serum of animals in the diagnosis of central or peripheral nervous system damage in veterinary medicine.
显示更多 [+] 显示较少 [-]Biomarkers of injury to neural tissue in veterinary medicine 全文
2016
Płonek Marta | Wrzosek Marcin | Nicpoń Józef
There are numerous biomarkers of central and peripheral nervous system damage described in human and veterinary medicine. Many of these are already used as tools in the diagnosis of human neurological disorders, and many are investigated in regard to their use in small and large animal veterinary medicine. The following review presents the current knowledge about the application of cell-type (glial fibrillary acidic protein, neurofilament subunit NF-H, myelin basic protein) and central nervous system specific proteins (S100B, neuron specific enolase, tau protein, alpha II spectrin, ubiquitin carboxy-terminal hydrolase L1, creatine kinase BB) present in the cerebrospinal fluid and/or serum of animals in the diagnosis of central or peripheral nervous system damage in veterinary medicine.
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2006
Choi, J.H. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Kim, H.J. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Kim, H.W. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Lee, E.C. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Jang, J.Y. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Ban, H.J. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Kim, J.K. (Haemaru Referral Animal Hospital, Seongnam, Republic of Korea) | Yoon, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
Caudal occipital malformation syndrome (COMS) is relatively common disease which occurs at craniocervical junction. It has been known that this syndrome was developed mainly in Cavalier King Charles spaniels, but these days there a lot of case reports about COMS in other small breed dogs. In this report, COMS was diagnosed in ten dogs presented to Haemaru Referral Animal Hospital using MRI examination from January 2005 to April 2006. These cases were also investigated about concurrent diseases and syringomyelia was the most common type of complication, but clinical signs were nonspecific.
显示更多 [+] 显示较少 [-]Detection of porcine encephalomyocarditis virus by in situ hybridization
1999
Oh, S.H. | Park, N.Y. | Chung, C.Y. | Cho, K.O. | Lee, B.J. | Park, Y.S. | Park, H.S. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
The purpose of this study was to establish a rapid, reliable diagnostic method detecting Encephalomyocarditis virus(EMCV) RNA in formalin-fixed, paraffin-embedded tissues of EMCV naturally infected pigs by cDNA probe of EMC K3, the EMCV strain isolated from Korea. Using a biotin-labelled nick translated probe for the cDNA marker. We made up for some defects of radiolabeled method. In situ hybridization(ISH) technique, differently from theother nucleic acid hybridization methods, is able to detect the virus genome specifically in the state of the intact shapes of cells and/or tissues. We succeeded in performing the experiment to detect the EMCV within 1~2 hours usign the MicroProbeTM capaillary action system. In this study, we ovserved highly specific positive sighals of red color by staining the paraffinembedded tissue sections of naturally EMCV-infected pig organs or tissues, including brain, heart, kidney and lacrimal gland with the Fast Red TR salt/Naphtol phosphate chromogen. The results suggested that this ISH method is considered as a highly sensitive and reliable tool for molecular biologic diagnosis of the EMC viral disease.
显示更多 [+] 显示较少 [-]Distribution of the neuropeptide Y immunoreactive neurons in the olfactory bulb of striped field mouse(Apodemus agrarius)
1999
Jeong, Y.G. | Lee, N.S. (Konyang University, Nonsan (Korea Republic). Department of Anatomy, College of Medicine) | Kim, K.S. (ASAN Institute for Life Science, Asan (Korea Republic). Department of Laboratory Animal Research) | Jung, J.Y. | Lee, K.Y. | Kim, M.K. (Chungnam National University, Taejon (Korea Republic). College of Veterinary Medicine)
This study was carried out to nvestigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into 40 micro meter-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae: and olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and alayer of white matter. The accessory olfactory bulb had four layers: an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region.
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