Small intestinal explants from weaned pigs were cultured under a variety of conditions. Explants maintained villus-to-crypt ratio between 1:1 and 1.5:1 for 48 hours. The mucosal epithelium remained well preserved and retained good cellular morphologic features, as determined by light and electron microscopy. Between 48 and 72 hours, considerable mucosal degeneration was evident. Best results were obtained when the explants were cultured on a rocking platform placed in an atmosphere of 95% O2 and 5% CO2, using supplemented RPMI 1640 cell culture medium.

Determination of the optimum parameters of cattle embryos production in vitro from high-producing cows after their slaughter on a meat-packing plant was realized in the conditions of the Republic of Belarus. Oocyte cumulus complexes of cows of black-motley breed and the conditions of their maturing were used as the object of experimental research. Oocyte cumulus complex were allocated with dissection of ovaries tissue placed into Hanks culture medium. The search and morphological estimation of quality of received oocyte cumulus complexes were realized by microscopic research. Then, the oocytes were placed into a culture medium for cell maturing in СО2-incubator at 38,5 deg C with the maximum humidity (98%), with presence of 5% СО2 under a layer of mineral oil for 24 hours. Matured oocytes were impregnated with the frozen-thawed sperm after capacitation. Oogenesis efficiency of production of pre-implantation germs in vitro was determined in accordance with the level of embryonic fission and production of viable germs. Use cumulus cells monolayer made it possible to increase the quantity of ripened oocytes on 5,2-5,7%, level of embryonic fission - on 14,0-14,6% and production of embryos - on 13,8-12,5% depending on a way of its production in comparison with groups of the cells which were cultivated without a monolayer. Thus, the research has shown the exploitability of use of cellular reproductive technologies in selection and breeding activities in cattle breeding. Their application in combination with embryo transplantation could make it possible to use the reproductive potential of high-priced oocyte donors more effectively for the genetic improvement of animal population efficiency