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Effects of ovarian storage condition on in vitro maturation of Hokkaido sika deer (Cervus nippon yesoensis) oocytes
2014
Tulake, K. ((Northeast Forestry University, Harbin (China). College of Wildlife Resources), (Xinjiang Agricultural University, Urumqi (China). College of Animal Science)) | Yanagawa, Y. | Takahashi, Y. | Katagiri, S. | Higaki, S. | Koyama, K. | Wang, X. | Li, H.
The follicle characteristic and immature oocyte quality obtained from repeated transvaginal oocyte retrieval in Bos indicus beef cows
2015
Izuan Bahtiar A. J. | Ahmad J. | Saadiah J. | Azizah A. | Ahmad Nazri A. R.
The development and availability of follicles is an indicator to predict which of the follicle sizes are suitable to recover the oocytes assisted by means of ultrasonography of transvaginal oocyte retrieval (OPU). Thus, the study was done in order to characterize the follicular recruitment and distribution in response to the repeated removal of follicles, and thus to determine the availability of follicles and immature oocytes harvested repeatedlyfor two consecutive days of OPU in beef cows. Results indicated that 24-h OPU showed significantly greater numbers of medium and large follicles than small categories (P<0.05). However the 48-hr of OPU does not showed any differences of follicles categories (P>0.05). The mean total number of follicles and immature oocytes recovered were higher (P<0.05) in 24-hr OPU (13.76±1.2 and 7.38 ± 1.7) compared to 48-hr OPU (9.08 ± 1.5 and 3.54 ± 1.00) with the oocyte retrieval rate of 51.22% and 38.17%, respectively. The morphological classification indicated the 24-hr oocyte retrieval produced 62% of suitable immature oocytes that can be used for in vitro embryo production. In conclusion, the repeated removal of two consecutive days of OPU has averted the development of dominant follicle, and thus, gave an atmosphere to the subordinate follicles to continue growth relatively to an equal proportion of small, medium and large categories of follicles. Due to the reduction of follicle and recovery rate at 48-hr it is suggested that OPU be carried out later than 48 hour so that the follicle has more time to increase the diameter size.
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