A study was conducted to determine the effect of different nutrients i.e. metabolizable energy (ME) and crude protein (CP) content of ration on the egg production and egg weight of rural layers. For the purpose six different experimental rations containing 2700, 2900 kcal/kg ME and 14, 16, 18 percent CP were offered to the birds. The results indicated that egg number increased with proportional adversely affected as the CP of the ration was increased weight of the layers was proportionally increased with ration at constant ME level, while increase in ME level egg weight. increase in ME, while it was at constant ME level. The egg increase in CP content of the did not significantly affect the egg weight.

This study was conducted to examine comparative productive performance of different varieties of Desi chickens identified from group of Desi flock procured from rural field conditions of Mianwali and Bhakkar districts of Punjab province (Pakistan). A total of five varieties of Desi chickens viz., dark brown, medium brown, light brown, black and mixed colour were identified on the basis of their plumage colour and uniformity of body characters. Four hundred and eighty birds (96 of each variety) were selected for further study. The birds of each variety were randomly divided into 3 equal replicates. They were maintained in 15 separate pens on deep litter system under optimal managemental conditions and were fed a balanced layers ration ad-libitum. The birds were provided 16 hours light per day. The experiment was conducted for 8 weeks. The results indicated that varieties differed significantly in egg production, feed conversion efficiency and egg mass, whereas, no difference was observed in feed intake and egg weight between them. Based on the findings of this study, it appeared that different varieties of Desi chickens identified and selected during the course of this study on phenotypic basis differed in their productive potential on the basis of better productive potentials in terms of egg yield, FCR and egg mass, dark brown, and black varieties of Desi chickens could be used to carry out further selective breeding work to study possibility of improving their productive potentials.

This study was conducted to ascertain anatomical variation in different body parts of Desi, Fayoumi, Cross (Rhode Island Red x Fayoumi) and Nick Chick laying hens. A total 16 laying hens of average body weight were selected using random numbers out of 2600 laying hens, slaughtered and eviscerated. It was observed that proportion of neck, ribs, breast, back, wings, thigh and legs out of aggregate weight was highest (51.74 plus minus 2.85) in Fayoumi hens. The proportion of liver, gizzard, heart and spleen combined weight was highest (6.05 plus minus 1.81) in desi hens. The proportion of non edible offals including trachea, lungs, kidneys, blood, feathers, head, crop, proventriculus, intestines, shanks, gizzard waste, skin, tail, testes, ova, oviduct and fat was estimated to be highest i.e. 46.60 plus minus 1.16 in Nick Chick laying hens. The anatomical variation in Desi and Fayoumi chicks was attributable to scavenging habit of these birds. The high proportion of edible offal in commercial Nick Chick hens may be ascribable to higher productive and reproductive traits.

This study was carried out for investigation of an outbreak in Karachi. A disease outbreak in poultry was reported during April, 2000 in Karachi. The main symptoms included respiratory distress, sneezing and gasping. Autopsy of dead birds showed tracheitis, air-sacculitis and involvement of lungs. The morbid material was collected for processing in the laboratory. During the investigation for isolation of pathogenic bacteria or virus, Ornithobacterium rhinotracheale was isolated on 5% sheep blood agar plates in an atmosphere containing 5-10% CO2 at 37 degree C and identified through biochemical and fermentation tests. A Lasota like virus was also isolated from the same material which showed HA activity but was found negative to Avian Influenza virus against known Avian Influenza serum. The isolated virus was sent to Central Veterinary Laboratory, Weybridge, U.K. which confirmed it to be Lasota virus having comparatively high Intracerebral Pathogenicity Index (ICPI) as 0.90. Attempts for the transmission of the disease in susceptible healthy broiler chicks was successful through aerosol route using combination of inoculum of the isolated bacteria and virus. On post-mortem of infected birds air- sacculitis, tracheitis and unilateral pneumonia were noticed. Again ORT and NDV were isolated from the material harvested from these chicks. According to the observations of these experiments, it was concluded that the isolated ORT had a triggering effect on Lasota virus or vice versa. The environmental temperature and other stress factors might have aggravated the disease problem.