Magnesium(Mg2+) is one of the most abundant intracellular divalent cation. Although recent studies demonstrate that adrenergic receptor stimulation evokes marked changes in Mg2+ homeostasis, the regulation of Mg2+ by dopaminergic receptor stimulation is not yet known. In this work, we uwed dopaminergic agents to identify which type(s) of receptors were involved inthe mobilization of Mg2+ by dopaminergic receptor stimulation in the perfused rat gearts, isolated myocytes and circulating blood. The mg2+ content was measured by atomic absorbance spedtrophotometry. Dopamine(DA), apomorphine(APO) and pergolide stimulated Mg2+ efflux in the perfused rat hearts and these effects were inhibited by haloperidol or fluphenazine, nonselective dopaminergic antagonists. SKF38393, a selective doparminergic agonist, increased Mg2+ efflux from the perfused hearts in dose dependant manners and SKF38393-induced Mg2+ efflux was potentiated in the presence of sulpiride or eticlopride, D2-selective antagonist, from the perfused hearts. This increase of Mg2+ efflux was blocked by haloperidol or imipramine. DA or pergolide increased in circulating Mg2+ from blood. By contrast, PPHT stimulated Mg2+ influx(a decrease in efflux) from the perfused hearts and circulating blood. PPHT-induced Mg2+ influx was blocked by fluphenazine in the perfused hearts. DA-stimulated Mg2+ efflux was inhibited by dopaminergic antagoinst in the isolated myocytes. In conclusion, the flux of Mg2+ is modulated by DA receptor activation in the rat hearts. The efflux of Mg2+ can be increased by D1-receptor stimulation and decreased by D2-receptor stimulation, respectively.

In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).