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Metagenomic analysis of acquired antibiotic resistance determinants in the gut microbiota of wild boars (Sus scrofa) – preliminary results
2020
Libisch, Balázs | Keresztény, Tibor | Kerényi, Zoltán | Kocsis, Róbert | Sipos, Rita | Papp, Péter P. | Olasz, Ferenc
Land application of manure that contains antibiotics and resistant bacteria may facilitate the establishment of an environmental reservoir of antibiotic-resistant microbes, promoting their dissemination into agricultural and natural habitats. The main objective of this study was to search for acquired antibiotic resistance determinants in the gut microbiota of wild boar populations living in natural habitats. Gastrointestinal samples of free-living wild boars were collected in the Zemplén Mountains in Hungary and were characterised by culture-based, metagenomic, and molecular microbiological methods. Bioinformatic analysis of the faecal microbiome of a hunted wild boar from Japan was used for comparative studies. Also, shotgun metagenomic sequencing data of two untreated sewage wastewater samples from North Pest (Hungary) from 2016 were analysed by bioinformatic methods. Minimum spanning tree diagrams for seven-gene MLST profiles of 104 E. coli strains isolated in Europe from wild boars and domestic pigs were generated in Enterobase. In the ileum of a diarrhoeic boar, a dominant E. coli O112ab:H2 strain with intermediate resistance to gentamicin, tobramycin, and amikacin was identified, displaying sequence type ST388 and harbouring the EAST1 toxin astA gene. Metagenomic analyses of the colon and rectum digesta revealed the presence of the tetQ, tetW, tetO, and mefA antibiotic resistance genes that were also detected in the gut microbiome of four other wild boars from the mountains. Furthermore, the tetQ and cfxA genes were identified in the faecal microbiome of a hunted wild boar from Japan. The gastrointestinal microbiota of the free-living wild boars examined in this study carried acquired antibiotic resistance determinants that are highly prevalent among domestic livestock populations.
显示更多 [+] 显示较少 [-]Report on the prevalence and experimental infections of Angiostrongylus siamensis Ohbayashi, Kamiya et Bhaibulaya, 1979, parasitic in the mesenteric arteries of rodents in Thailand
1980
Kamiya, M. | Oku, Y. | Katakura, K. | Kamiya, H. | Ohbayashi, M. | Abe, H. | Suzuki, H. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Three trichostrongylid nematodes from the red rock rabbits, Pronolagus sp., in Kenya
1980
Fukumoto, S. | Kamiya, M. | Suzuki, H. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Preliminary application of a formalin fixed tissue section to the indirect fluorescent antibody test and intraoral precipitin reaction for the diagnosis of Schistosomiasis japonica
1980
Kamiya, H. | Kamiya, M. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
