细化搜索
结果 1-10 的 30
Evaluation of Genetic Diversity in Ross 308 Broiler Chicken using LEI0258 Microsatellite Marker 全文
2022
Vatankhah, Afra | Nikbakhat Brujeni, Gholamraza | Esmailnejad, Atefeh | Mirzai, Parisa
BACKGROUND: Major histocompatibility complex (MHC) encodes for highly variable molecules, most of which are responsible for foreign antigen recognition and activation of immune responses in the host. LEI0258 microsatellite, located in the poultry MHC region, is a suitable genetic marker for determining MHC haplotypes and genetic diversity in poultry.OBJECTIVES: Considering the fact that there is no report on the frequency and types of MHC alleles and population genetic analysis in Ross 308 poultry in Iran, the present study aimed to investigate the diversity of MHC haplotypes of Ross 308 broilers by LEI0258 microsatellite.METHODS: A total of 216 blood samples were collected from two productive herds of Ross 308 broilers. After extracting DNA of the blood samples and amplifying LEI0258 microsatellite alleles, genotyping of MHC haplotypes was performed using agarose gel electrophoresis and fragment analysis techniques.RESULTS: A total of seven alleles and 21 genotypes were identified for LEI0258 microsatellite in these two groups. the highest and the lowest frequencies belonged respectively to allele 385 bp (42.86 %) and allele 300 bp (4.33 %). Heterozygous 207/385 was found to be the dominant genotype in both populations. According to the similarity matrix analysis, there was an 84.56 % similarity between the two groups.CONCLUSIONS: The results obtained in this study revealed a high level of heterozygosity (85.71 % and 91.35 %) and deviation from Hardy–Weinberg equilibrium (P<0.0001) in these two Ross populations. Ross 308 broiler chickens had lower allelic diversity and higher genetic similarity compared to the native ones. These findings provided additional information on the use of MHC as a candidate gene marker in genetic improvement and resource conservation in broiler populations.
显示更多 [+] 显示较少 [-]Study of BuLA-DRB3 polymorphism in Khuzestan river buffaloes 全文
2016
Ranjbar, Mohammad Mehdi | Nikbakhat Brujeni, Gholamraza | Ghadrdan Mashhadi, Alireza | Dabbaghyan, Mehran
BACKGROUND: Major histocompatibility complex (MHC) comprises a group of genes, which plays a central role in immune response. The exon 2 of BuLA-DRB3 is part of the MHC class II in buffalo that highly polymorphic, found to be associated with resistance/susceptibility to infections and also with production parameters. OBJECTIVES: The purpose of the present study is to identify BuLA-DRB3 polymorphism in Khuzestan buffaloes and compare this population with other Iranian and world buffalo populations. METHODS: Blood samples were taken from 136 unrelated Khuzestan river buffaloes. After DNA extraction, second exon of BuLA-DRB3 was amplified by the seminested PCR method. Then, the fragments produced by amplifying second exon were cut by RsaI restriction enzyme according to van Eijik method. In the following, allelic frequencies, genotype frequencies, expected and observed homozygosty and heterozygosity were calculated. RESULTS: In restriction fragment analysis 13 and 24 different allelic and genotype patterns were identified for RsaI restriction enzyme, respectively. 10 out of 13 alleles were previously reported. The most frequent genotype was oo)0.1691) and then followed by hh (0.1544) ,ll(0.1103), lw (0.0955), lh (0,0808), ha (0.0661) and lo (0.0514). Also four most frequent alleles were o (0.2721), h (0.2316),l (0.2316)and w (0.1176), respectively. These seven genotypes and four alleles form 72.76% and74.29% overall genotype and allele frequency of population. In addition, estimation of heterozygosity/homozygosty and deviation from Hardy-Weinberg equilibriumofcorresponding population revealed observed homozygosty is more than heterozygosity and departure of population from Hardy-Weinberg equilibrium. CONCLUSIONS: The results indicated that exon 2 of the BuLA-DRB3 gene is highly polymorphic among Khuzestan buffaloes and although, there is differences between buffalo’s genetic polymorphism of distinct world regions, Khuzestan buffaloes’ population is similar to Egyptian buffaloes’ population.
显示更多 [+] 显示较少 [-]Study of Single Nucleotide Polymorphisms of Major Histocompatibility Complex Region Related to the Immune System in Commercial Broiler and Layer Chickens 全文
2019
Pish Jang Aghajeri, Jafar | Rahimi Mianji, Ghodrat | Hafezian, Seyyed Hassan | Gholizadeh, Mohsen
BACKGROUND: Chicken major histocompatibility complex region (MHC) is important in the productive traits, immune responses, resistance to infectious diseases and phylogenetic relationships. OBJECTIVES: This study was investigated for single nucleotide polymorphisms of MHC region related to the immune system in commercial broiler and layer chickens. METHODS: One hundred blood samples were taken from commercial broiler and layer chickens and genomic DNA was extracted by salting out method. The allelic polymorphisms were investigated in B-L, B-F and B-G loci using PCR-RFLP and MspI enzyme. RESULTS: For two commercial broiler and laying populations, in the 374 bp locus of B-L, only BB genotype was detected but in the 1048 bp locus of B-F, two genotypes of CG and GG were identified in broiler chickens. The C allele contained four bands of 515, 410, 75 and 47 bp, and the G allele with five bands of 410, 302, 213, 75 and 47 bp. In B-G (401 bp) locus, three genotypes of MM, MN and NN and two alleles of M with one band (401 bp) and N with two bands (350 and 51 bp) were identified. In total populations, the Shannon information index was calculated to be 0.45 and 0.73 in markers loci of B-F and B-G, and the fixation index values were -0.20 and 0.34, respectively. The highest observed heterozygosity index for B-F and B-G loci was 0.34 and 0.23, respectively. CONCLUSIONS: Considering the confirmation of the presence of polymorphism in two loci of the B-F (in commercial broiler population) and B-G (in commercial broiler and layer populations), these sites can be used as genetic marker in breeding programs to increase resistance to diseases.
显示更多 [+] 显示较少 [-]A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads 全文
2017
Nieto Farias, María Victoria | Caffaro, María Eugenia | Lendez, Pamela Anahí | Passucci, Juan | Poli, Mario Andres | Ceriani, María Carolina | Dolcini, Guillermina Laura
A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads 全文
2017
Nieto Farias, María Victoria | Caffaro, María Eugenia | Lendez, Pamela Anahí | Passucci, Juan | Poli, Mario Andres | Ceriani, María Carolina | Dolcini, Guillermina Laura
Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures. | O vírus da leucemia bovina (BLV) está associado à doença neoplásica mais comum do gado bovino. O BLV tem uma disseminação silenciosa no rebanho devido à troca de células infectadas, assim, a concentração de células BLV infectadas no sangue deve desempenhar um papel importante no sucesso da transmissão viral. Os genes do antígeno leucocitário bovino (BoLA), sistema MHC do gado bovino, estão associados à resistência genética e à susceptibilidade a uma ampla gama de doenças, bem como às características da produção. Alguns polimorfismos de alelos de BoLA DRB3.2 em bovinos Holstein têm sido associados à resistência ou susceptibilidade ao desenvolvimento da doença BLV, ou com carga proviral (PVL). Esta investigação avaliou 107 vacas leiteiras da raça Holstein argentina infectadas com BLV e pertencentes a um único rebanho. A PVL foi analisada por qPCR, os animais foram classificados em alta carga proviral (HPVL, N = 88) e baixa carga proviral (LPVL, N = 19), e os alelos BoLA DRB3.2 foram genotipados. Os alelos BoLA DRB3.2*1501 e *1201 estavam significativamente relacionados à HPVL (p = 0,0230 e p = 0,0111, respectivamente), enquanto o alelo BoLA DRB3.2*0201, à LPVL (p = 0,0030). O objetivo deste estudo é contribuir para o conhecimento da associação entre o polimorfismo de BoLA e o desenvolvimento de infecção por BLV. Os genes que melhor explicam a PVL na população analisada resultaram em BoLA DRB3.2*0201 (como fator de proteção) e *1501 (como fator de risco). As diferenças alélicas podem desempenhar um papel importante no desenvolvimento de respostas imunitárias eficazes. Uma melhor compreensão de como o polimorfismo BoLA contribui para estas respostas e o estabelecimento de um estado BLV é desejável para agendar e avaliar as medidas de controle. | Inst. de Genética "Ewald A. Favret"- IGEAF | Fil: Nieto Farias, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina | Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | Fil: Lendez, Pamela Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina | Fil: Passucci, Juan. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Epidemiología; Argentina | Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | Fil: Ceriani, María Carolina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina | Fil: Dolcini, Guillermina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina
显示更多 [+] 显示较少 [-]A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads 全文
2017
María Victoria Nieto Farias | María Eugenia Caffaro | Pamela Anahí Lendez | Juan Passucci | Mario Poli | María Carolina Ceriani | Guillermina Laura Dolcini
Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures.
显示更多 [+] 显示较少 [-]A novel association of BoLA DRB3 alleles with BLV infected cattle with different proviral loads | Uma nova associação de alelos de BoLA DRB3 em bovinos infectados com BLV com diferentes cargas provirais 全文
2017
Nieto Farías, María Victoria | Caffaro, María Eugenia | Lendez, Pamela Anahí | Passucci, Juan Antonio | Poli, Mario Andres | Ceriani, Maria Carolina | Dolcini, Guillermina Laura
Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures. | O vírus da leucemia bovina (BLV) está associado à doença neoplásica mais comum do gado bovino. O BLV tem uma disseminação silenciosa no rebanho devido à troca de células infectadas, assim, a concentração de células BLV infectadas no sangue deve desempenhar um papel importante no sucesso da transmissão viral. Os genes do antígeno leucocitário bovino (BoLA), sistema MHC do gado bovino, estão associados à resistência genética e à susceptibilidade a uma ampla gama de doenças, bem como às características da produção. Alguns polimorfismos de alelos de BoLA DRB3.2 em bovinos Holstein têm sido associados à resistência ou susceptibilidade ao desenvolvimento da doença BLV, ou com carga proviral (PVL). Esta investigação avaliou 107 vacas leiteiras da raça Holstein argentina infectadas com BLV e pertencentes a um único rebanho. A PVL foi analisada por qPCR, os animais foram classificados em alta carga proviral (HPVL, N = 88) e baixa carga proviral (LPVL, N = 19), e os alelos BoLA DRB3.2 foram genotipados. Os alelos BoLA DRB3.2*1501 e *1201 estavam significativamente relacionados à HPVL (p = 0,0230 e p = 0,0111, respectivamente), enquanto o alelo BoLA DRB3.2*0201, à LPVL (p = 0,0030). O objetivo deste estudo é contribuir para o conhecimento da associação entre o polimorfismo de BoLA e o desenvolvimento de infecção por BLV. Os genes que melhor explicam a PVL na população analisada resultaram em BoLA DRB3.2*0201 (como fator de proteção) e *1501 (como fator de risco). As diferenças alélicas podem desempenhar um papel importante no desenvolvimento de respostas imunitárias eficazes. Uma melhor compreensão de como o polimorfismo BoLA contribui para estas respostas e o estabelecimento de um estado BLV é desejável para agendar e avaliar as medidas de controle. | Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina | Fil: Lendez, Pamela Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Passucci, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina | Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina | Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
显示更多 [+] 显示较少 [-]Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production 全文
2013
Makhsous Samaneh Gorji | Mirhoseini Seyed Ziaeddin | Zamiri Mohammad Javad | Niazi Ali
Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production 全文
2013
Makhsous Samaneh Gorji | Mirhoseini Seyed Ziaeddin | Zamiri Mohammad Javad | Niazi Ali
A total of 142 chicken blood samples were collected and a specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with SacI and MspI restriction endonucleases. The amplified fragment digested with SacI enzyme revealed two “+” (wild type) and “-” (normal type) alleles with the frequency of 0.898 and 0.102, respectively. The amplified fragment digested with MspI enzyme revealed three A, B and C alleles with the frequency of 0.599, 0.102, and 0.299, respectively. Frequencies of +/+, +/- and -/- were 0.817, 0.162, and 0.021, respectively, and those of AA, AB, AC, BB, BC, and CC were 0.338, 0.113, 0.409, 0.007, 0.070, and 0.063, respectively, in the studied population. The results of 2 and likelihood ratio tests showed that this population was at Hardy-Weinberg equilibrium with respect to the marker locus. Marker-trait association analysis revealed statistically significant differences between “SacI-RFLP” genotypes for egg production and rate of laying eggs. The relationship between the molecular marker and these traits can be useful to improve the chicken breeding programmes.
显示更多 [+] 显示较少 [-]Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production 全文
2013
Makhsous, Samaneh Gorji | Mirhoseini, Seyed Ziaeddin | Zamiri, Mohammad Javad | Niazi, Ali
A total of 142 chicken blood samples were collected and a specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with SacI and MspI restriction endonucleases. The amplified fragment digested with SacI enzyme revealed two “+” (wild type) and “-” (normal type) alleles with the frequency of 0.898 and 0.102, respectively. The amplified fragment digested with MspI enzyme revealed three A, B and C alleles with the frequency of 0.599, 0.102, and 0.299, respectively. Frequencies of +/+, +/- and -/- were 0.817, 0.162, and 0.021, respectively, and those of AA, AB, AC, BB, BC, and CC were 0.338, 0.113, 0.409, 0.007, 0.070, and 0.063, respectively, in the studied population. The results of 2 and likelihood ratio tests showed that this population was at Hardy-Weinberg equilibrium with respect to the marker locus. Marker-trait association analysis revealed statistically significant differences between “SacI-RFLP” genotypes for egg production and rate of laying eggs. The relationship between the molecular marker and these traits can be useful to improve the chicken breeding programmes.
显示更多 [+] 显示较少 [-]INVESTIGATION THE POLYMORPHISM OF BONE MORPHOGENETIC PROTEIN 15 (BMP-15) GENE IN IRAQ COW 全文
2016
Hayder AL-Mutar
Bone Morphogenetic Protein 15 (BMP-15) was known to regulate functions of ovaryin mammals. The aim of this research was tocompare the BMP15 expression ofovaries incow and calf todetermine a relationship between the level of BMP15 genesand the developmental low competence of calfoocytes.Oocyte collected from 15cattleand 10 calves. Extracted DNA by intron kit (Korea).The BMP15 expression inOocytes surrounded completely andpartiallyby cumulus cells of adult ovaries washigher significantly than that incalf ovaries. Oocytepolymorphism in protein calvescompared with adults
显示更多 [+] 显示较少 [-]DNA polymorphism analysis of hereditary multiple exostoses in horses
1989
Li, J.K.K. | Moloney, B.K. | Shupe, J.L. | Gardner, E.J. | Leone, N.C. | Elsner, Y.
Genomic DNA polymorphisms obtained by restriction fragment-length polymorphism from healthy horses and horses with hereditary multiple exostoses were analyzed. These DNA were digested by 12 restriction enzymes and were hybridized against 6 isotopically labeled oncogene probes. Hybridization was not detected with the viral oncogene, v-ras, which indicated this oncogene was absent in the equine genome. Oncogenes (c-raf-1, c-fes, c-myb, c-myc, and c-sis) were present and had similar hybridization patterns and signal intensities in DNA from healthy horses and horses with hereditary multiple exostoses. Unique and distinct restriction fragment-length polymorphisms were detected with the c-raf-1 probe only in BamHI- and PstI-digested equine DNA.
显示更多 [+] 显示较少 [-]Genetic Diversity Assessment in Iraqi Local Goat Breeds by Using Molecular Markers 全文
2023
Awat Yousif | Mohammed Abdalla
Goats play a significant role in the economy of Iraq through livestock production, income generation, employment opportunities, and the conservation of valuable genetic resources. Genetic diversity of three Iraqi local goat breeds were studied by using two molecular markers, fifteen microsatellite (SSR) and fifteen RAP DNA markers. Individual blood samples were collected and individual genomic DNA were extracted from 30 Black, 10 Hybrid, and 20 Meriz goat breeds. PCR amplification was conducted. The results revealed that out of 15 SSR primers, 11 were amplified and showed 847 total bands, 53 were polymorphic with 6.58 percentage of polymorphic bands. All the fifteen RAPD primers amplified 6085 total bands, in which 273 were polymorphic bands with 4.33 percentage of polymorphic bands. Different unique bands were detected for each breed. Both SSR and RAPD gave moderate polymorphism 66.67% and 61.52%, respectively. Besides, this value was consistent with the moderate value of the mean of polymorphism information content 0.19 and 0.28, respectively. Meriz and Hybrid breeds revealed the longest genetic distance (0.114 and 0.316). While, Black and Meriz breeds revealed the highest closeness (0.956 and 0.831) for SSR and RAPD markers, respectively. Furthermore, the UPGMA dendrogram for both of SSR and RAPD markers classified the three goat breeds into two main clusters. The first one contained Black and Hybrid breeds. While, the second one contained only Meriz breed. The results of the current study will be helpful for future researchers as a key guide to better understanding the genetic relationships and breed differences in Iraqi goat breeds for planning strategies for the future genetic improvement program.
显示更多 [+] 显示较少 [-]GENETIC POLYMORPHISM AND DIVERSITY OF IRAQI AWASSI SHEEP USING PCR-RAPD TECHNIQUE 全文
2020
Zainab S. Al-Allak | Maytham A. Dragh | Ahmed Sadoon Hussain
The establishment of modern sheep production systems in Iraq, lead to presence of variousforms of hybridization between the native and Middle East breeds which have been utilized forgenetic improvement. This occur in consistence with the progressive destruction or deteriorationof sheep habitat. Together, these factors have accelerated the loss of genetic diversity or evenresulted in the extinction of some indigenous breeds. Therefore, it is important to developefficient strategies for surveillance, evaluation, conservation and utilization of the availablegenetic resources for this species. Seven random amplification polymorphism DNA (RAPD)marker used. The aim of this study was to assess genetic diversity for Awassi native breed inIraq. The higher polymorphism information contents at the seven markers (Seventy- three bandsobtained with 28.3% of polymorphism) indicate the retention of natural variation from sourcepopulations for the domestic breeds of different geographic regions in Iraq. Analysis of geneticdifferentiation revealed substantial divergence among these breeds as 16% diversity indicatingthat some evolutionary forces (e.g. selection and migration, uncontrolled selling across borders)had acted on these populations. Phylogenetic and phylogeographic analyses displayed aremarkable degree of consistency between geographic origins, breeding histories and the patternof genetic differentiation.
显示更多 [+] 显示较少 [-]IDENTIFICATION OF LANCIFIELD SEROGROUP G STREPTOCOCCUS CANIS BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS (PCR-RFLP) OF 16S RIBOSOMAL RNA GENE 全文
2013
Abdulwahed Ahmed Hassan
In this study S. canis and 12 various species and serogroups of streptococci including: S. agalactiae, S. dysgalactiae subsp. dysgalactiae (serogroup C and L), S. dysgalactiae subsp. equisimilis (serogroup G), S. uberis, S. parauberis, S. phocae, S. suis, S. equi subsp. equi, S. equi subsp. zooepidimicus, S. porcinus and S. pyogenes were used and identified reliably by PCR-restriction fragment length polymorphism analysis (PCR-RFLP) of 1.43 kb of 16S ribosomal RNA gene using universal oligonuclotide primers and subsequent digestion with the restriction endonucleases including RsaI, MspI and AvaII. The PCR-RFLP results showed that RsaI restriction RFLP pattern of S. canis appeared different with all streptococci species baring the S. equi subsp. equi and S. equi subsp. zooepidimicus. The MspI restriction RFLP pattern of S. canis could be differentiated from S. agalactiae, S. dysgalactiae subsp. dysgalactiae (serogroups C and L), S. dysgalactiae subsp. equisimilis (serogroups G), S. phocae, S. suis, S. porcinus and S. pyogenes. The AvaII restriction RFLP pattern of S. canis could be distinguished from S. dygalactiae subsp. dysgalactiae (serogroup C and L), S. dygalactiae subsp. equisimilis (serogroup G) S. parauberis, S. phocae and S. suis. In conclusion, PCR-RFLP method using restriction endonucleases RsaI, MspI and AvaII could be useful method for identification of S. canis from S. dysgalactiae subsp. dysgalactiae (serogroup C and L), S. dysgalactiae subsp. equisimilis (serogroup G) and other related streptococci species. It can be concluded that RFLP method might help to determine the prevalence of S. canis in animal and human infections .
显示更多 [+] 显示较少 [-]