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Immunohistochemistry for detection of Aujeszky's disease virus antigens: Protein A-gold labelling of ultrathin sections for electron microscopy
1989
Kim, S.B. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veterinary Medicine)
The present study was carried out to determine viral antigens and its morphogenesis in the ultrathin frozen and araldite sections of cell cultures infected with ADV by protein A-gold labelling. ADV antigens were labelled with 10nm gold probes, and electron-dense gold particles were mainly present on viral nucleocapsids and viral envelopes. Immunogold labelling in the ultracryosections showed a very low degree of interaction with tissue structures. Immunogold labelling in the ultrathin cryosections can be useful tool for the detection of ADV antigens, and the technique also may provide its great potential for immunocytochemical studies on various virus-host cell interactions
显示更多 [+] 显示较少 [-]Serotypes of staphylococcus hyicus subsp hyicus isolated from pigs
1989
Kim, D.K. | Yeo, S.G. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veterinary Medicine)
Serotypes of S hyicus subsp hyicus strains isolated from healthy pigs and piglets with exudative epidermitis were identified with homogeneous factor sera. The serotypes of 489 strains were classified into 5 serotypes as A (16.8 %), C (20.4 %), D (9.4 %), E (7.2 %), F (29.2 %) and untypable (17.0 %). Serotype F was the most predominant strain regardless of health condition, body regions and age of the pigs
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