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A survey of gastrointestinal parasitic infection on small ruminant farms in Seberang Perai Selatan
2018
Zulkarnain Mazukhi | Jamaiyah Mat Isa | Shahaza Othman | Aishya Han | Khor, S. K. | Syamsyul Azizan | Suhaimi Ali | Saipul Bahari Abdul Ree
In Malaysia, helminthiasis due to strongyles such as Haemonchuscontortus and coccidiosis caused by Eimeria sp. have been reported to cause severe economic losses in small ruminants livestock industry. This paper reports the occurrence of gastrointestinal parasite infections on small ruminants situated in Seberang Perai Selatan district, Penang. Faecal samples were obtained from a total of 193 animals,randomly selected from 14 ruminant farms. The results of this survey indicates that helminthiasis and coccidiosis is rampant insheep and goat farms. The most common infections diagnosed were helminthiasis (77.72%) and coccidiosis (60.10%) followedby Moniezia sp. (5.18%). From this study, it shows that parasitic diseases can be managed by good animal husbandryin farms since high parasitic infections were observed in farms that were poorly managed based on nutrition, hygiene andbasic animal husbandry practices. The smallholders depended on health and extension services from the State Veterinary Department. A continuous monitoring of small ruminant farms by the Department of Veterinary Services will provide important information for assisting farmers with managing the spread of parasitic infections and maintaining the productivity of animals.
显示更多 [+] 显示较少 [-]Assessment of genetic diversity in Malin sheep using microsatellite markers
2018
Suriaty R. | Mohd Hafiz A. R. | Halimaton Sa’adiah T. | Zawawi I.
Malin sheep is the indigenous sheep breed of Malaysia and mainlykept for meat production. A total of 48 individuals from the National Institute of Veterinary Biodiversity (NIVB) in Jerantut,Pahang were used. The objective of this study was to assess the genetic diversity in the Malin using microsatellite markers.Eleven microsatellite loci were successfully amplified in 48 Malin sheep. All loci were polymorphic. A total of 66 alleles were detected. The number of observed alleles per locus varied from 12 to 21, with mean observed number alleles per locus of15.18±4.58. The observed heterozygosity and expected heterozygosity were 0.0189±0.01 and 0.8989±0.01, respectively. The mean polymorphic information content (PIC) value was 0.8970±0.01, indicating that the used markers were highly informative and could be used in parentage identification. Tests of genotype frequencies for deviation from the Hardy-Weinberg equilibrium (HWE), at each locus revealed depature from HWE due to loss in heterozygotes by high levels of inbreeding. The average inbreeding value for the 11 markers investigated was0.9797±0.01 indicating a more homozygous nature of the population. This is the first report of microsatelitte based variations in Malin sheep breed and can be useful for development of a rational breeding strategy for genetic improvement of sheepin Malaysia which may benefit future conservation programmes.
显示更多 [+] 显示较少 [-]Monitoring of Brucella sp., Coxiella burnetii and aflatoxin M1 in goat milk from Johor
2018
Sarol K. | Putri Nur Atifi M. N. | Saudah A. | Ketty, G. S. L. | Khairunnisak M. | Faridah I. | Nursyuhada M. R.
Forty-nine fresh goat’s milk samples produced by local farmers and sold in market for public consumption as well as raw goat milk in Johor, Malaysia were analysed for total plate count(TPC) , E. coli, Coliform, Brucella melitensis, Brucella abortus,Coxiella burnetii as well as aflatoxin M1 (AFM1) content, as measures for food safety. The mean counts per ml for TPC were 4.90 x 105, 6.50 x 105, 1.60 x 105 and 1.48 x 106 for pasteurised, unpasteurised and unknown (status of pasteurisation) milk sold in the market as well as the raw milk from milkcollection center (MCC), respectively. Among pasteurised samples, only one had TPC count higher than the permitted level whereas the rest were all within the permitted level. The mean counts per ml for E. coli were <1.00 x 102 for pasteurised and unknown milkwhereas 1.67 x 101 for unpasteurised and 1.18 x 102 for raw milk. The mean counts per ml for coliform were 9.53 x 103, 9.76 x103, 1.20 x 102 and 1.16 x 104 for pasteurised, unpasteurised, unknown milk and raw milk, respectively. Overall, no significantdifferences on the bacterial counts in both pasteurised and unpasteurised milk. All milk samples were negative of B. melitensis and B. abortus, but one unknown sample fromthe market and two raw samples from MCC were positive of C. burnetii through the ELISA test. The unknown sample from the market showed the presence of C. burnetii when further analysed microscopically. Meanwhile, no sample exceeded the permitted level of AFM1 in milk.
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