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A study was carried out in 2161 quarter milk samples of 550 cows in Durg district Chhattisgarh. Out of 550 animals, 385 (70%) animals were found to be positive for sub clinical mastitis (SCM) by Modified White Side Test (MWST), 432 (78.54%) by Modified California Mastitis Test (MCMT) and 462 (84%) by somatic cell count (SCC). The quarter wise prevalence of sub clinical mastitis was 47.99%, 55.25% and 60.90% by MWST, MCMT and SCC respectively. Prevalence of blind teats was 1.77%. prevalence was highest during second and third lactations and at 5 and 6 years of age. Infection rate was higher during early and late stages of lactation. HF and Jersey cross bred cows were more susceptible than indigenous cows. Microorganisms isolated were predominantly Staphylococci. ABST revealed sensitivity to cefotaxime whereas most of the isolates were resistant to ampicillin.

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